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Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation

Title
Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation
Authors
Kim M.S.Lee S.Yun S.Suh P.-G.Park J.Cui M.Choi S.Cha S.-S.Jin W.
Ewha Authors
차선신
SCOPUS Author ID
차선신scopus
Issue Date
2018
Journal Title
International Journal of Biological Macromolecules
ISSN
0141-8130JCR Link
Citation
vol. 107, pp. 1650 - 1658
Keywords
DJ-1Inorganic phosphateParkinson's diseaseROSTartrate
Publisher
Elsevier B.V.
Indexed
SCI; SCIE; SCOPUS scopus
Abstract
The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (Pi)-dependent manner in vitro. Here, we demonstrated that Pi and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced Pi- and ROS-induced DJ-1 aggregation and restored Pi- and ROS-provoked cell death using quantitative data as mean ± standard deviation, and statistics. Mechanistically, tartrate prevented DJ-1 aggregation via occupying the Pi-binding site. These findings revealed an unexpected physiological role of tartrate in the maintenance of DJ-1 function, and thus, a potential use as an inhibitor of DJ-1 aggregation. © 2017 Elsevier B.V.
DOI
10.1016/j.ijbiomac.2017.10.022
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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