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Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation
- Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation
- Kim M.S.; Lee S.; Yun S.; Suh P.-G.; Park J.; Cui M.; Choi S.; Cha S.-S.; Jin W.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- International Journal of Biological Macromolecules
- vol. 107, pp. 1650 - 1658
- DJ-1; Inorganic phosphate; Parkinson's disease; ROS; Tartrate
- Elsevier B.V.
- SCI; SCIE; SCOPUS
- The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (Pi)-dependent manner in vitro. Here, we demonstrated that Pi and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced Pi- and ROS-induced DJ-1 aggregation and restored Pi- and ROS-provoked cell death using quantitative data as mean ± standard deviation, and statistics. Mechanistically, tartrate prevented DJ-1 aggregation via occupying the Pi-binding site. These findings revealed an unexpected physiological role of tartrate in the maintenance of DJ-1 function, and thus, a potential use as an inhibitor of DJ-1 aggregation. © 2017 Elsevier B.V.
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