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Concanavalin A Induces Cortical Neuron Apoptosis by Causing ROS Accumulation and Tyrosine Kinase Activation

Title
Concanavalin A Induces Cortical Neuron Apoptosis by Causing ROS Accumulation and Tyrosine Kinase Activation
Authors
Jang S.Yayeh T.Leem Y.-H.Park E.-M.Ito Y.Oh S.
Ewha Authors
오세관박은미
SCOPUS Author ID
오세관scopus; 박은미scopus
Issue Date
2017
Journal Title
Neurochemical Research
ISSN
0364-3190JCR Link
Citation
vol. 42, no. 12, pp. 3504 - 3514
Keywords
Concanavalin AIntracellular ROSNeuronal apoptosisTyrosine kinase
Publisher
Springer New York LLC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The lectin, concanavalin A (Con A), is the most extensively investigated member of the lectin family of plant proteins, but its effects on cortical neurons and astrocytes are poorly understood. In cultured cortical neurons and astrocytes, Con A exhibited dose-dependent neurotoxicity, but this was not observed in astrocytes. Similarly, in the cortical areas of rat brains, intracranial administration of Con A caused neuronal but no astrocyte damage. Methyl-α-d-mannopyranoside, a competitor of Con A, blocked Con A-induced cell death, whereas AMPA/KA receptor antagonists showed partial blocking effects. Furthermore, the mRNA levels of TNF-α, IL-1β, and IL-6 were elevated in astrocytes and cortical neurons treated with Con A. Intracellular reactive oxygen species (ROS) levels were increased in Con A-treated cortical neurons, and N-acetyl-cysteine (NAC, an antioxidant) and diphenyleneiodonium (DPI, a NADPH oxidase inhibitor) reduced intracellular ROS accumulation. Likewise, AG556 (a TNF-α inhibitor) and AG82 (a tyrosine kinase inhibitor) both reduced Con A-induced intracellular ROS accumulation. Furthermore, Con A-induced tyrosine phosphorylation was decreased by NAC and by AG556. Taken together, Con A-induced apoptosis in cortical neurons occurred as a sequel to Con A binding to neuronal glycoproteins and intracellular ROS accumulation. Interestingly, Con A-induced cellular damage was observed in cortical neurons but not in astrocytes or microglia. © 2017, Springer Science+Business Media, LLC.
DOI
10.1007/s11064-017-2398-2
Appears in Collections:
의과대학 > 의학과 > Journal papers
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