View : 2 Download: 0

Galangin Suppresses Pro-Inflammatory Gene Expression in Polyinosinic-Polycytidylic Acid-Stimulated Microglial Cells

Title
Galangin Suppresses Pro-Inflammatory Gene Expression in Polyinosinic-Polycytidylic Acid-Stimulated Microglial Cells
Authors
Choi, Min-JiPark, Jin-SunPark, Jung-EunKim, Han SuKim, Hee-Sun
Ewha Authors
김희선김한수
SCOPUS Author ID
김희선scopus; 김한수scopus
Issue Date
2017
Journal Title
BIOMOLECULES & THERAPEUTICS
ISSN
1976-9148JCR Link2005-4483JCR Link
Citation
vol. 25, no. 6, pp. 641 - 647
Keywords
GalanginPoly(I:C)MicrogliaPI3K/AktNF-kappa BPPAR-gamma signaling
Publisher
KOREAN SOC APPLIED PHARMACOLOGY
Indexed
SCIE; SCOPUS; KCI WOS
Abstract
Galangin (3,5,7-trihydroxyflavone) is a polyphenolic compound abundant in honey and medicinal herbs, such as Alpinia officinarum. In this study, we investigated the anti-inflammatory effects of galangin under in vitro and in vivo neuroinflammatory conditions caused by polyinosinic-polycytidylic acid (poly(I:C)), a viral mimic dsRNA analog. Galangin suppressed the production of nitric oxide, reactive oxygen species, and pro-inflammatory cytokines in poly(I:C)-stimulated BV2 microglia. On the other hand, galangin enhanced anti-inflammatory interleukin (IL)-10 production. Galangin also suppressed the expression of pro-inflammatory markers in poly(I:C)-injected mouse brains. Further mechanistic studies showed that galangin inhibited poly(I:C)-induced nuclear factor (NF)-kappa B activity and phosphorylation of Akt without affecting MAP kinases. Interestingly, galangin increased the expression and transcriptional activity of peroxisome proliferator-activated receptor (PPAR)-gamma, known to play an anti-inflammatory role. To investigate whether PPAR-gamma is involved in the anti-inflammatory function of galangin, BV2 cells were pre-treated with PPAR-gamma antagonist before treatment of galangin. We found that PPAR-gamma antagonist significantly blocked galangin-mediated upregulation of IL-10 and attenuated the inhibition of tumor necrosis factor (TNF)-alpha and IL-6 in poly(I:C)-stimulated microglia. In conclusion, our data suggest that PI3K/Akt, NF-kappa B, and PPAR-gamma play a pivotal role in mediating the anti-inflammatory effects of galangin in poly(I:C)-stimulated microglia.
DOI
10.4062/biomolther.2017.173
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE