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ω-hydroxyundec-9-enoic acid induces apoptosis by ROS mediated JNK and p38 phosphorylation in breast cancer cell lines

Title
ω-hydroxyundec-9-enoic acid induces apoptosis by ROS mediated JNK and p38 phosphorylation in breast cancer cell lines
Authors
Ahn J.Chung Y.W.Park J.-B.Yang K.M.
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2018
Journal Title
Journal of Cellular Biochemistry
ISSN
0730-2312JCR Link
Citation
vol. 119, no. 1, pp. 998 - 1007
Keywords
anti-cancer effectapoptosisJNK pathwayp38 pathwayROSω-Hydroxyundec-9-enoic acid
Publisher
Wiley-Liss Inc.
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
ω-Hydroxyundec-9-enoic acid (ω-HUA), a plant secondary metabolite, exhibits anti-fungal activity. However, its effect on breast cancer cells is unknown. Here, we investigated the anti- breast cancer activity of ω-HUA and its underlying mechanism. Treatment of human breast cancer cell lines, MDA-MB-231 and MDA-MB-435, with ω-HUA induced apoptotic cell death with increased cleaved caspase-3 and poly (ADP-ribose) polymerase (PARP) levels, and p38 and JNK phosphorylation. Inhibition of these mitogen-activated protein kinase (MAPK) pathways using specific inhibitors or siRNA, for p38 and JNK, respectively, blocked the ω-HUA-induced apoptosis in a dose-dependent manner. Moreover, pretreatment of the cells with antioxidant N-acetyl cysteine (NAC) inhibited ω-HUA-induced increased reactive oxygen species (ROS) levels, cleaved caspase-3 and cleaved PARP, and phosphorylated JNK, phosphorylated p38, and increased cell viability and colony-forming ability. MDA-MB-231 xenograft model showed that the ω-HUA-treated group exhibited greater tumor regression and significantly reduced tumor weight compared to that exhibited by the vehicle-administered group. Collectively, ω-HUA-induced intracellular ROS generation induced breast cancer cell apoptosis through JNK and p38 signaling pathway activation, resulting in tumor regression. The results suggested that ω-HUA is an effective supplement for inhibiting human breast cancer growth. © 2017 Wiley Periodicals, Inc.
DOI
10.1002/jcb.26267
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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