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Longitudinal Monitoring of Antibody Responses against Tumor Cells Using Magneto-nanosensors with a Nanoliter of Blood

Title
Longitudinal Monitoring of Antibody Responses against Tumor Cells Using Magneto-nanosensors with a Nanoliter of Blood
Authors
Lee, Jung-RokChan, Carmel T.Ruderman, DanielChuang, Hui-YenGaster, Richard S.Atallah, MichelleMallick, ParagLowe, Scott W.Gambhir, Sanjiv S.Wang, Shan X.
Ewha Authors
이정록
Issue Date
2017
Journal Title
NANO LETTERS
ISSN
1530-6984JCR Link1530-6992JCR Link
Citation
vol. 17, no. 11, pp. 6644 - 6652
Keywords
Magneto-nanosensorsbioluminescent imagingmagnetic nanoparticleslymphomacancer immunologyimmunoglobulin isotypes
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magneto-resistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum. Combining in vitro serologic measurements with in vivo imaging techniques, we investigated the role of the antibody response in the regression of firefly luciferase (FL)-labeled lymphoma cells in spleen, kidney, and lymph nodes in a syngeneic Burkitt's lymphoma mouse model. Regression status was determined by whole body bioluminescent imaging (BLI). The magneto-nanosensors revealed that anti-FL IgG2a and total IgG2a were elevated and sustained in regression mice compared to non-regression mice (p < 0.05). This platform shows promise for monitoring immunotherapy, vaccination, and autoimmunity.
DOI
10.1021/acs.nanolett.7b02591
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엘텍공과대학 > 휴먼기계바이오공학부 > Journal papers
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