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Longitudinal Monitoring of Antibody Responses against Tumor Cells Using Magneto-nanosensors with a Nanoliter of Blood
- Title
- Longitudinal Monitoring of Antibody Responses against Tumor Cells Using Magneto-nanosensors with a Nanoliter of Blood
- Authors
- Lee, Jung-Rok; Chan, Carmel T.; Ruderman, Daniel; Chuang, Hui-Yen; Gaster, Richard S.; Atallah, Michelle; Mallick, Parag; Lowe, Scott W.; Gambhir, Sanjiv S.; Wang, Shan X.
- Ewha Authors
- 이정록
- SCOPUS Author ID
- 이정록
- Issue Date
- 2017
- Journal Title
- NANO LETTERS
- ISSN
- 1530-6984
1530-6992
- Citation
- NANO LETTERS vol. 17, no. 11, pp. 6644 - 6652
- Keywords
- Magneto-nanosensors; bioluminescent imaging; magnetic nanoparticles; lymphoma; cancer immunology; immunoglobulin isotypes
- Publisher
- AMER CHEMICAL SOC
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Each immunoglobulin isotype has unique immune effector functions. The contribution of these functions in the elimination of pathogens and tumors can be determined by monitoring quantitative temporal changes in isotype levels. Here, we developed a novel technique using magneto-nanosensors based on the effect of giant magneto-resistance (GMR) for longitudinal monitoring of total and antigen-specific isotype levels with high precision, using as little as 1 nL of serum. Combining in vitro serologic measurements with in vivo imaging techniques, we investigated the role of the antibody response in the regression of firefly luciferase (FL)-labeled lymphoma cells in spleen, kidney, and lymph nodes in a syngeneic Burkitt's lymphoma mouse model. Regression status was determined by whole body bioluminescent imaging (BLI). The magneto-nanosensors revealed that anti-FL IgG2a and total IgG2a were elevated and sustained in regression mice compared to non-regression mice (p < 0.05). This platform shows promise for monitoring immunotherapy, vaccination, and autoimmunity.
- DOI
- 10.1021/acs.nanolett.7b02591
- Appears in Collections:
- 공과대학 > 휴먼기계바이오공학과 > Journal papers
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