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TRPV3 Channel in Keratinocytes in Scars with Post-Burn Pruritus

Title
TRPV3 Channel in Keratinocytes in Scars with Post-Burn Pruritus
Authors
Park, Chun WookKim, Hyun JiChoi, Yong WonChung, Bo YoungWoo, So-YounSong, Dong-KeunKim, Hye One
Ewha Authors
우소연
SCOPUS Author ID
우소연scopus
Issue Date
2017
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1422-0067JCR Link
Citation
vol. 18, no. 11
Keywords
protease-activated receptor 2transient receptor potential vanniloid 3thymic stromal lymphopoietinpost-burn pruritus
Publisher
MDPI AG
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Post-burn pruritus is a common and distressing sequela of burn scars. Empirical antipruritic treatments usually fail to have a satisfactory outcome because of their limited selectivity and possible side effects. Therefore, novel drug targets need to be identified. Here, we aimed to investigate the possible role of protease-activated receptor 2 (PAR2) and transient receptor potential vanniloid 3 (TRPV3), along with the relation of TRPV3 to thymic stromal lymphopoietin (TSLP). Specimens from normal (unscarred) or burn-scarred (with or without pruritus) tissue were obtained from burn patients for this study. In each sample, the keratinocytes were isolated and cultured, and the intracellular Ca2+ level at the time of stimulation of each factor was quantified and the interaction was screened. PAR2 function was reduced by antagonism of TRPV3. Inhibiting protein kinase A (PKA) and protein kinase C (PKC) reduced TRPV3 function. TSLP mRNA and protein, and TSLPR protein expressions, increased in scars with post-burn pruritus, compared to scars without it or to normal tissues. In addition, TRPV1 or TRPV3 activation induced increased TSLP expression. Conclusively, TRPV3 may contribute to pruritus in burn scars through TSLP, and can be considered a potential therapeutic target for post-burn pruritus.
DOI
10.3390/ijms18112425
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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