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Fundamental limit of alpha-synuclein pathology in gastrointestinal biopsy as a pathologic biomarker of Parkinson's disease: Comparison with surgical specimens

Title
Fundamental limit of alpha-synuclein pathology in gastrointestinal biopsy as a pathologic biomarker of Parkinson's disease: Comparison with surgical specimens
Authors
Shin C.Park S.-H.Yun J.Y.Shin J.H.Yang H.-K.Lee H.-J.Kong S.-H.Suh Y.-S.Shen G.Kim Y.Kim H.-J.Jeon B.
Ewha Authors
윤지영
SCOPUS Author ID
윤지영scopusscopus
Issue Date
2017
Journal Title
Parkinsonism and Related Disorders
ISSN
1353-8020JCR Link
Citation
vol. 44, pp. 73 - 78
Keywords
Alpha-synucleinBiopsyGastrointestinal tractImmunohistochemistryParkinson's diseaseSurgical specimen
Publisher
Elsevier Ltd
Indexed
SCIE; SCOPUS scopus
Abstract
Objective Alpha-synuclein (AS) accumulation identified by immunohistochemistry (IHC) of gastrointestinal (GI) tract biopsies is considered as a potential pathologic biomarker for Parkinson's disease (PD). We compared AS IHC findings in biopsy specimens and surgically resected full-depth specimens to examine the reliability of GI tract biopsies. Methods We included patients with PD who had undergone operation of the GI tract for treatment of tumors. Controls were matched with age at operation, gender, and surgical resection site. We compared AS accumulation using phosphorylated AS (pAS) IHC between patients and controls, and within individuals between surgical and biopsy specimens. Results A total of 33 patients with PD were categorized into either the stomach (N = 12) or colorectal group (N = 21). The frequency of pAS positivity in gastric surgical specimens was 58.3% (7/12) and 8.3% (1/12) in the patient and control groups, respectively (p = 0.027). The frequency of pAS positivity in colorectal surgical specimens was identical in the patient and control group (23.8% [5/21] in each). Intriguingly, immunostaining results for biopsy specimens were not concordant with those for surgical specimens. There was no significant difference in the frequency of pAS positivity in biopsy specimens between patients and controls (9.1% [2/22] vs 18.2% [4/22]; p = 0.664). Interpretation Our results demonstrate that AS accumulation identified via pAS IHC of GI biopsies is unreliable due to its low positive rates and poor concordance with surgical specimens, and that future studies investigating AS accumulation in the GI tract should target the stomach, rather than the colon or rectum. © 2017
DOI
10.1016/j.parkreldis.2017.09.001
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의료원 > 의료원 > Journal papers
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