View : 71 Download: 0

Enhancement of lysine production in recombinant Corynebacterium glutamicum through expression of Deinococcus radiodurans pprM and dr1558 genes

Title
Enhancement of lysine production in recombinant Corynebacterium glutamicum through expression of Deinococcus radiodurans pprM and dr1558 genes
Authors
Kim S.-M.Lim S.Park S.J.Joo J.C.Choi J.-I.
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2017
Journal Title
Microbiology and Biotechnology Letters
ISSN
2234-7305JCR Link
Citation
vol. 45, no. 3, pp. 271 - 275
Keywords
Corynebacterium glutamicumDR1558LysinePprM
Publisher
Korean Society for Microbiolog and Biotechnology
Indexed
SCOPUS scopus
Abstract
The expression of Deinococcus radiodurans dr1558 and pprM genes was examined for enhanced lysine production in recombinant Corynebacterium glutamicum. These genes are known to confer high tolerance to pH and osmotic shock in Escherichia coli. D. radiodurans dr1558 and pprM genes were expressed in C. glutamicum by using 6 synthetic promoters of different strengths, to evaluate the effect of expression efficiency on lysine production. Recombinant C. glutamicum expressing DR1558 under the L26 and I64 promoters showed higher lysine production than that expressing DR1558 under other promoters. Similarly, recombinant C. glutamicum expressing PprM under same promoters (L26 and I64) showed a higher increase in lysine production compared to that expressing PprM under other promoters. In the absence of CaCO3 in the medium, the expression of DR1558 or PprM also increased lysine concentration in C. glutamicum depending on the promoter used. Together, these results suggest that genes involved in radiation tolerance in D. radiodurans can be used to enhance production of amino acids and their derivatives. © 2017, The Korean Society for Microbiology and Biotechnology.
DOI
10.4014/mbl.1708.08003
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE