Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박시재 | * |
dc.date.accessioned | 2017-12-28T16:30:29Z | - |
dc.date.available | 2017-12-28T16:30:29Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 1860-6768 | * |
dc.identifier.issn | 1860-7314 | * |
dc.identifier.other | OAK-21733 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/239550 | - |
dc.description.abstract | The authors previously reported the production of polyhydroxyalkanoates (PHAs) containing 2-hydroxyacid monomers by expressing evolved Pseudomonas sp. 6-19 PHA synthase and Clostridium propionicum propionyl-CoA transferase in engineered microorganisms. Here, the authors examined four butyryl-CoA transferases from Roseburia sp., Eubacterium hallii, Faecalibacterium prausnitzii, and Anaerostipes caccae as potential CoA-transferases to support synthesis of polymers having 2HA monomer. In vitro activity analyses of the four butyryl-CoA transferases suggested that each butyryl-CoA transferase has different activities towards 2-hydroxybutyrate (2HB), 3-hydroxybutyrate (3HB), and lactate (LA). When Escherichia coli XL1-Blue expressing Pseudomonas sp. 6-19 PhaC1437 along with one butyryl-CoA transferase is cultured in chemically defined MR medium containing 20 g L-1 of glucose, 2 g L-1 of sodium 3-hydroxybutyrate, and various concentrations of sodium 2-hydroxybutyrate, PHAs consisting of 3HB, 2HB, and LA are produced. The monomer composition of PHAs agreed well with the substrate specificities of butyryl-CoA transferases from E. hallii, F. prausnitzii, and A. caccae, but not Roseburia sp. When E. coli XL1-Blue expressing PhaC1437 and E. hallii butyryl-CoA transferase is cultured in MR medium containing 20 g L-1 of glucose and 2g L-1 of sodium 2-hydroxybutyrate, P(65.7 mol% 2HB-co-34.3 mol% LA) is produced with the highest PHA content of 30 wt%. Butyryl-CoA transferases also supported the production of P(3HB-co-2HB-co-LA) from glucose as the sole carbon source in E. coli XL1-Blue strains when one of these bct genes is expressed with phaC1437, cimA3.7, leuBCD, panE, and phaAB genes. Butyryl-CoA transferases characterized in this study can be used for engineering of microorganisms that produce PHAs containing novel 2-hydroxyacid monomers. | * |
dc.language | English | * |
dc.publisher | WILEY-V C H VERLAG GMBH | * |
dc.subject | butyryl-CoA transferase | * |
dc.subject | polyhydroxyalkanoates (PHAs) | * |
dc.subject | 2-hydroxyacids | * |
dc.subject | P(3-hydroxybutyrate-co-2-hydroxybutyrate-co-lactate) | * |
dc.subject | Escherichia coli | * |
dc.title | Biosynthesis of 2-Hydroxyacid-Containing Polyhydroxyalkanoates by Employing butyryl-CoA Transferases in Metabolically Engineered Escherichia coli | * |
dc.type | Article | * |
dc.relation.issue | 11 | * |
dc.relation.volume | 12 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | BIOTECHNOLOGY JOURNAL | * |
dc.identifier.doi | 10.1002/biot.201700116 | * |
dc.identifier.wosid | WOS:000419207600007 | * |
dc.identifier.scopusid | 2-s2.0-85033451214 | * |
dc.author.google | David, Yokimiko | * |
dc.author.google | Joo, Jeong Chan | * |
dc.author.google | Yang, Jung Eun | * |
dc.author.google | Oh, Young Hoon | * |
dc.author.google | Lee, Sang Yup | * |
dc.author.google | Park, Si Jae | * |
dc.contributor.scopusid | 박시재(57191670770) | * |
dc.date.modifydate | 20240322131024 | * |