Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 심현보 | * |
dc.date.accessioned | 2017-12-28T16:30:27Z | - |
dc.date.available | 2017-12-28T16:30:27Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 1064-3745 | * |
dc.identifier.other | OAK-21739 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/239544 | - |
dc.description.abstract | Rabbits have distinct advantages over mice as a source of target-specific antibodies. They produce higher affinity antibodies than mice, and may elicit strong immune response against antigens or epitopes that are poorly immunogenic or tolerated in mice. However, a great majority of currently available monoclonal antibodies are of murine origin because of the wider availability of murine fusion partner cell lines and well-established tools and protocols for fusion and cloning of mouse hybridoma. Phage-display selection of antibody libraries is an alternative method to hybridoma technology for the generation of target-specific monoclonal antibodies. High-affinity monoclonal antibodies from nonmurine species can readily be obtained by constructing immune antibody libraries from B cells of the immunized animal and screening the library by phage display. In this article, we describe the construction of a rabbit immune Fab library for the facile isolation of rabbit monoclonal antibodies. After immunization, B-cell cDNA is obtained from the spleen of the animal, from which antibody variable domain repertoires are amplified and assembled into a Fab repertoire by PCR. The Fab genes are then cloned into a phagemid vector and transformed to E. coli, from which a phage-displayed immune Fab library is rescued. Such a library can be biopanned against the immunization antigen for rapid identification of high-affinity, target-specific rabbit monoclonal antibodies. © Springer Science+Business Media LLC 2018. | * |
dc.language | English | * |
dc.publisher | Humana Press Inc. | * |
dc.subject | Antibody library | * |
dc.subject | Fab library | * |
dc.subject | Immune antibody library | * |
dc.subject | Phage display | * |
dc.subject | Rabbit antibody | * |
dc.subject | Rabbit monoclonal antibody | * |
dc.title | Construction of rabbit immune antibody libraries | * |
dc.type | Book Chapter | * |
dc.relation.volume | 1701 | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 133 | * |
dc.relation.lastpage | 146 | * |
dc.relation.journaltitle | Methods in Molecular Biology | * |
dc.identifier.doi | 10.1007/978-1-4939-7447-4_7 | * |
dc.identifier.scopusid | 2-s2.0-85033661931 | * |
dc.author.google | Nguyen T.T.H. | * |
dc.author.google | Lee J.S. | * |
dc.author.google | Shim H. | * |
dc.contributor.scopusid | 심현보(26635827900) | * |
dc.date.modifydate | 20240123110611 | * |