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Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process

Title
Production of 5-aminovaleric acid in recombinant Corynebacterium glutamicum strains from a Miscanthus hydrolysate solution prepared by a newly developed Miscanthus hydrolysis process
Authors
Kang, Kyoung HeeSong, Bong KeunJoo, Jeong ChanOh, Young HoonYu, Ju HyunHyun, Sung MinKhang, Tae UkPark, KyungmoonOh, Min-KyuLee, Sang YupPark, Si Jae
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2017
Journal Title
BIORESOURCE TECHNOLOGY
ISSN
0960-8524JCR Link1873-2976JCR Link
Citation
vol. 245, pp. 1692 - 1700
Keywords
Corynebacterium glutamicumMetabolic engineering5-Aminovaleric acidMiscanthusPretreatment
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
This study examined nine expired industrial Corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-AVA. C. glutamicum KCTC 1857 exhibiting the highest lysine production was transformed with either original Pseudomonas putida davBA genes, encoding the 5-AVA biosynthesis pathway, or C. glutamicum codon-optimized davBA genes. C. glutamicum KCTC 1857 expressing the original genes had superior cell viability and 5-AVA production capability compared to the other strain. This strain produced 39.93 g/L of 5-AVA, which is the highest titer reported to date in fed-batch fermentation from glucose. Indeed, Miscanthus hydrolysate solution prepared from a novel process, comprising pretreatment, hydrolysis, purification, and concentration, was used as feedstock for 5-AVA production. A total of 12.51 g/L 5-AVA was produced from the Miscanthus hydrolysate; this value is 34.7% higher than that obtained from glucose in batch fermentation. (C) 2017 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.biortech.2017.05.131
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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