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Engineering the xylose-catabolizing Dahms pathway for production of poly(d-lactate-co-glycolate) and poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) in Escherichia coli

Title
Engineering the xylose-catabolizing Dahms pathway for production of poly(d-lactate-co-glycolate) and poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) in Escherichia coli
Authors
Choi S.Y.Kim W.J.Yu S.J.Park S.J.Im S.G.Lee S.Y.
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2017
Journal Title
Microbial Biotechnology
ISSN
1751-7907JCR Link
Citation
vol. 10, no. 6, pp. 1353 - 1364
Publisher
John Wiley and Sons Ltd
Indexed
SCOPUS WOS scopus
Abstract
Poly(lactate-co-glycolate), PLGA, is a representative synthetic biopolymer widely used in medical applications. Recently, we reported one-step direct fermentative production of PLGA and its copolymers by metabolically engineered Escherichia coli from xylose and glucose. In this study, we report development of metabolically engineered E. coli strains for the production of PLGA and poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) having various monomer compositions from xylose as a sole carbon source. To achieve this, the metabolic flux towards Dahms pathway was modulated using five different synthetic promoters for the expression of Caulobacter crescentus XylBC. Further metabolic engineering to concentrate the metabolic flux towards d-lactate and glycolate resulted in production of PLGA and poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) with various monomer fractions from xylose. The engineered E. coli strains produced polymers containing 8.8–60.9 mol% of glycolate up to 6.93 g l−1 by fed-batch cultivation in a chemically defined medium containing xylose. Finally, the biocompatibility of poly(d-lactate-co-glycolate-co-d-2-hydroxybutyrate) was confirmed by live/dead assay using human mesenchymal stem cells. © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
DOI
10.1111/1751-7915.12721
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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