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Recombinant baculovirus-based vaccine expressing M2 protein induces protective CD8+ T-cell immunity against respiratory syncytial virus infection

Title
Recombinant baculovirus-based vaccine expressing M2 protein induces protective CD8+ T-cell immunity against respiratory syncytial virus infection
Authors
Lee J.-Y.Chang J.
Ewha Authors
장준
SCOPUS Author ID
장준scopus
Issue Date
2017
Journal Title
Journal of Microbiology
ISSN
1225-8873JCR Link
Citation
vol. 55, no. 11, pp. 900 - 908
Keywords
cd8 t cellsm2 proteinrecombinant baculovirusrespiratory syncytial virusvaccine
Publisher
Microbiological Society of Korea
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Respiratory syncytial virus (RSV) is an important cause of acute lower respiratory tract disease in infants, young children, immunocompromised individuals, and the elderly. However, despite ongoing efforts to develop an RSV vaccine, there is still no authorized RSV vaccine for humans. Baculovirus has attracted attention as a vaccine vector because of its ability to induce a high level of humoral and cellular immunity, low cytotoxicity against various antigens, and biological safety for humans. In this study, we constructed a recombinant baculovirus- based vaccine expressing the M2 protein of RSV under the control of cytomegalovirus promoter (Bac_RSVM2) to induce CD8+ T-cell responses which play an important role in viral clearance, and investigated its protective efficacy against RSV infection. Immunization with Bac_RSVM2 via intranasal or intramuscular route effectively elicited the specific CD8+ T-cell responses. Most notably, immunization with Bac_RSVM2 vaccine almost completely protected mice from RSV challenge without vaccine-enhanced immunopathology. In conclusion, these results suggest that Bac_RSVM2 vaccine employing the baculovirus delivery platform has promising potential to be developed as a safe and novel RSV vaccine that provides protection against RSV infection. © 2017, The Microbiological Society of Korea and Springer-Verlag GmbH Germany.
DOI
10.1007/s12275-017-7306-6
Appears in Collections:
약학대학 > 약학과 > Journal papers
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