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IL-10 Plays a Pivotal Role in Tamoxifen-Induced Spasmolytic Polypeptide-Expressing Metaplasia in Gastric Mucosa

Title
IL-10 Plays a Pivotal Role in Tamoxifen-Induced Spasmolytic Polypeptide-Expressing Metaplasia in Gastric Mucosa
Authors
Lee, ChansuLee, HyukHwang, Seo YunMoon, Chang MoHong, Sung Noh
Ewha Authors
문창모
SCOPUS Author ID
문창모scopus
Issue Date
2017
Journal Title
GUT AND LIVER
ISSN
1976-2283JCR Link2005-1212JCR Link
Citation
vol. 11, no. 6, pp. 789 - 797
Keywords
Stomach neoplasmsSpasmolytic polypeptide-expressing metaplasiaInterleukin-10
Publisher
EDITORIAL OFFICE GUT & LIVER
Indexed
SCIE; SCOPUS; KCI WOS
Abstract
Background/Aims: Gastric cancer evolves in the pathologic mucosal milieu, and its development is characterized by both the loss of acid-secreting parietal cells and mucosal cell metaplasia, called spasmolytic polypeptide-expressing metaplasia (SPEM). Cytokines, such as interleukin (IL)-10, IL-1 beta, and IL-6, play a key role in gastric carcinogenesis. However, changes in the cytokine profile of SPEM have not been evaluated. Methods: To induce SPEM in mouse stomachs, C57BL/6 mice were intraperitoneally injected with tamoxifen and sacrificed at 3, 10, and 21 days after treatment. RNA-sequencing (RNA-seq) and a multiplex bead array were used to measure cytokines in the stomachs of tamoxifen-treated/control mice. Results: The administration of tamoxifen led to the rapid development and histological normalization of SPEM 3 and 10 days after administration, respectively. RNA-seq revealed that the expression of IL-10 was decreased 3 days after tamoxifen administration. The multiplex assay identified a significant decline in IL-10 levels 3 days after tamoxifen treatment (58.38 +/- 34.44 pg/mL vs 94.09 +/- 4.98 pg/mL, p=0.031), which normalized at 10 and 21 days after tamoxifen treatment. Immunofluorescence staining confirmed that IL-10 expression was markedly decreased at the time of SPEM development and subsequently returned to normal, accompanied by a reversal in histologic changes. Conclusions: IL-10 may play a pivotal role in the tamoxifen-induced acute development of gastric SPEM.
DOI
10.5009/gnl16454
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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