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Identification of calcium/calmodulin-dependent phosphatase as the dephosphorylating enzyme of IgE-dependent histamine-releasing factor in RBL-2H3

Title
Identification of calcium/calmodulin-dependent phosphatase as the dephosphorylating enzyme of IgE-dependent histamine-releasing factor in RBL-2H3
Authors
Hwang S.-O.Lee K.
Ewha Authors
이경림
SCOPUS Author ID
이경림scopus
Issue Date
2005
Journal Title
Korean Journal of Microbiology and Biotechnology
ISSN
1598-642XJCR Link
Citation
vol. 33, no. 3, pp. 189 - 193
Indexed
SCOPUS; KCI scopus
Abstract
IgE-dependent histamine-releasing factor(HRF) was initially described as a secretagogue for secretion of histamine from IgE+ basophils from a subset of allergic donors. Previously, we identified that S98 residue of HRF was phosphorylated using anti-HRFpS98 antibody which specifically recognizes the phosphorylated serine residue of HRF and HRFS98A mutant construct. In vitro kinase assay, only wild type HRF was phosphorylated by PKC, and S98A HRF was not affected by PKC. In this study, we attempted to characterize the phosphatase which specifically dephosphorylates HRF by immunoprecipitation and pull-down assay. In RBL-2H3 cells, HRF interacted only with calcineurin (also called as PP2B, calcium/calmodulin-dependent phosphatase) but not with PP1 or PP2A. The results suggest that HRF is most likely dephosphorylated by calcineurin.
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약학대학 > 약학과 > Journal papers
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