View : 19 Download: 0

Simvastatin treatment boosts benefits of apoptotic cell infusion in murine lung fibrosis

Title
Simvastatin treatment boosts benefits of apoptotic cell infusion in murine lung fibrosis
Authors
Lee, Ye-JiKim, Meung-JooYoon, Young-SoChoi, Youn-HeeKim, Hee-SunKang, Jihee Lee
Ewha Authors
이지희김희선
SCOPUS Author ID
이지희scopus; 김희선scopus
Issue Date
2017
Journal Title
CELL DEATH & DISEASE
ISSN
2041-4889JCR Link
Citation
vol. 8
Publisher
NATURE PUBLISHING GROUP
Indexed
SCIE; SCOPUS WOS
Abstract
A single early-phase infusion of apoptotic cells can inhibit bleomycin-induced lung inflammation and fibrosis; however, it is unknown whether these effects can be enhanced with additional infusions and/or statin treatment. Here, we investigated whether an increased frequency of apoptotic cell injection, with or without efferocytosis enhancer simvastatin, facilitates therapeutic efficacy. An additional injection of apoptotic cells during the intermediate phase (7 days post-bleomycin treatment) or simvastatin administration alone on days 7-13 post-treatment did not promote anti-fibrotic responses beyond those induced by a single early apoptotic cell infusion alone. Additional administration of apoptotic cells with simvastatin further enhanced the efferocytic ability of alveolar macrophages and PPAR. activity, and induced hepatocyte growth factor and interleukin-10 expression, in alveolar macrophages and lung tissue. Additional administration of apoptotic cells with simvastatin also reduced mRNA expression of bleomycin-induced epithelial-mesenchymal transition (EMT) markers in isolated alveolar type II epithelial cells, fibrotic markers in fibroblasts, and hydroxyproline in lung tissue. Enhanced anti-EMT and anti-fibrotic efficacy was confirmed by immunofluorescence and trichrome staining of lung tissue. This suggests that additional administration of apoptotic cells with simvastatin during the intermediate phase of bleomycin-induced lung fibrosis may boost the anti-fibrotic properties of early apoptotic cell infusion.
DOI
10.1038/cddis.2017.260
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE