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Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes

Title
Lipolytic efficacy of alginate double-layer nanoemulsion containing oleoresin capsicum in differentiated 3T3-L1 adipocytes
Authors
Lee, Mak-SoonJung, SunyoonShin, YoonjinLee, SeohyunKim, Chong-TaiKim, In-HwanKim, Yangha
Ewha Authors
김양하
SCOPUS Author ID
김양하scopus
Issue Date
2017
Journal Title
FOOD & NUTRITION RESEARCH
ISSN
1654-6628JCR Link1654-661XJCR Link
Citation
vol. 61
Keywords
Free fatty acidglycerollipolysismRNA expressionobesity
Publisher
TAYLOR &; FRANCIS LTD
Indexed
SCIE; SCOPUS WOS
Abstract
Background: Oleoresin capsicum (OC) is an organic extract from fruits of the genus Capsicum, and has been reported to have an anti-obesity effect. Objective: This study comparatively investigated lipolytic effects of single-layer nanoemulsion (SN) and alginate double-layer nanoemulsion (AN) containing OC in 3T3-L1 adipocytes. Methods: SN and AN were compared by analyzing the intracellular lipid accumulation, triglyceride (TG) content, release of free fatty acids (FFAs) and glycerol, and mRNA expression of genes related to adipogenesis and lipolysis were analyzed in fully differentiated 3T3-L1 adipocytes. Results: Compared with SN, AN exhibited higher efficiency in inhibiting the intracellular lipid accumulation and TG content, and enhanced the release of FFAs and glycerol into the medium. In AN-treated cells, mRNA levels of peroxisome proliferator-activated receptor-gamma and the fatty acid-binding protein adipocyte protein-2, which are involved in adipogenesis, were down-regulated, whereas those of genes related to lipolysis, including hormone-sensitive lipase and carnitine palmitoyl transferase-1 alpha, were up-regulated compared with SN-treated cells. Conclusion: The lipolytic effect of AN was greater than that of SN; this was partly associated with the increased TG hydrolysis via induction of lipolytic gene expression and suppression of adipogenic gene expression in 3T3-L1 adipocytes.
DOI
10.1080/16546628.2017.1339553
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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