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Intracellular Remodeling and Accumulation of Aberrant Lysosomes in Differentiation of Tonsil-Derived Mesenchymal Stem Cells into Parathyroid-Like Cells

Title
Intracellular Remodeling and Accumulation of Aberrant Lysosomes in Differentiation of Tonsil-Derived Mesenchymal Stem Cells into Parathyroid-Like Cells
Authors
Jo Y.-I.Kim G.Jin Y.M.Park Y.J.Kim H.S.Park Y.S.
Ewha Authors
김한수
SCOPUS Author ID
김한수scopus
Issue Date
2017
Journal Title
Tissue Engineering and Regenerative Medicine
ISSN
1738-2696JCR Link
Citation
vol. 14, no. 4, pp. 411 - 420
Keywords
AutophagyMultilamellar bodiesMultivesicular bodiesParathyroid-like differentiationTonsil-derived mesenchymal stem cells
Publisher
Korean Tissue Engineering and Regenerative Medicine Society
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Differentiation of mesenchymal stem cells (MSC) into a variety of cell lineages such as adipocytes, osteocytes, and chondrocytes is often accompanied up-regulation of autophagy. In our study, we demonstrated that the expression of autophagy-associated proteins (p-Beclin 1, LC3A, LC3B, p-AMPK, p-mTOR and ATG3, ATG7, and ATG12-5) over a period of time was hardly distinguishable from control tonsil-derived MSC (TMSC). Despite the unnoticeable difference in autophagy activation between differentiated TMSC (dTMSC) and the control (cTMSC), we reported significant changes in intracellular compositions in differentiated TMSC into functional parathyroid-like cells secreting parathyroid hormone (PTH). By using transmission electron microscopy (TEM), we observed accumulation of multivesicular bodies (MVB) comprising small, degraded compartments densely accumulated as dark granular or amorphous clumps, multilamellar bodies and lipid droplets in dTMSC. However, no such structures were found in cTMSC. These results suggest that differentiation of TMSC into parathyroid-like cells producing PTH hormone is hardly dependent on autophagy activation in the beginning of our conditions. Furthermore, our results of intracellular remodeling and accumulated endo-lysosomal storage bodies in the later stages of TMSC differentiation present a possible role of the structures in PTH secretion. © 2017, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.
DOI
10.1007/s13770-017-0042-5
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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