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dc.contributor.author이수영-
dc.contributor.author이진규-
dc.contributor.author나윤철-
dc.date.accessioned2017-10-27T11:45:13Z-
dc.date.available2017-10-27T11:45:13Z-
dc.date.issued2017-
dc.identifier.issn0003-2670-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/237138-
dc.description.abstractA sensitive and selective capillary electrophoresis-mass spectrometry (CE-MS) method for determination of saturated fatty acids (FAs) was developed by using dicationic ion-pairing reagents forming singly charged complexes with anionic FAs. For negative ESI detection, 21 anionic FAs at pH 10 were separated using ammonium formate buffer containing 40% acetonitrile modifier in normal polarity mode in CE by optimizing various parameters. This method showed good separation efficiency, but the sensitivity of the method to short-chain fatty acids was quite low, causing acetic and propionic acids to be undetectable even at 100 mgL−1 in negative ESI-MS detection. Out of the four dicationic ion-pairing reagents tested, N,N’-dibutyl 1,1’-pentylenedipyrrolidium infused through a sheath-liquid ion source during CE separation was the best reagent regarding improved sensitivity and favorably complexed with anionic FAs for detection in positive ion ESI-MS. The monovalent complex showed improved ionization efficiency, providing the limits of detection (LODs) for 15 FAs ranging from 0.13 to 2.88 μg/mL and good linearity (R2 > 0.99) up to 150 μg/mL. Compared to the negative detection results, the effect was remarkable for the detection of short- and medium-chain fatty acids. The optimized CE-paired ion electrospray (PIESI)-MS method was utilized for the determination of FAs in cheese and coffee with simple pretreatment. This method may be extended for sensitive analysis of unsaturated fatty acids. © 2017 Elsevier B.V.-
dc.languageEnglish-
dc.publisherElsevier B.V.-
dc.subjectCapillary electrophoresis-mass spectrometry (CE-MS)-
dc.subjectIon paring reagent-
dc.subjectPaired ion electrospray ionization (PIESI)-
dc.subjectSaturated fatty acid-
dc.titleSaturated fatty acid determination method using paired ion electrospray ionization mass spectrometry coupled with capillary electrophoresis-
dc.typeArticle-
dc.relation.volume984-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage223-
dc.relation.lastpage231-
dc.relation.journaltitleAnalytica Chimica Acta-
dc.identifier.doi10.1016/j.aca.2017.06.052-
dc.identifier.wosidWOS:000408360600021-
dc.identifier.scopusid2-s2.0-85023172734-
dc.author.googleLee J.-H.-
dc.author.googleKim S.-J.-
dc.author.googleLee S.-
dc.author.googleRhee J.-K.-
dc.author.googleLee S.Y.-
dc.author.googleNa Y.-C.-
dc.contributor.scopusid이수영(7409697278)-
dc.contributor.scopusid이진규(56599780300)-
dc.date.modifydate20180517105427-
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자연과학대학 > 생명과학전공 > Journal papers
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