Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이수영 | * |
dc.contributor.author | 이진규 | * |
dc.contributor.author | 나윤철 | * |
dc.date.accessioned | 2017-10-27T11:45:13Z | - |
dc.date.available | 2017-10-27T11:45:13Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 0003-2670 | * |
dc.identifier.other | OAK-21135 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/237138 | - |
dc.description.abstract | A sensitive and selective capillary electrophoresis-mass spectrometry (CE-MS) method for determination of saturated fatty acids (FAs) was developed by using dicationic ion-pairing reagents forming singly charged complexes with anionic FAs. For negative ESI detection, 21 anionic FAs at pH 10 were separated using ammonium formate buffer containing 40% acetonitrile modifier in normal polarity mode in CE by optimizing various parameters. This method showed good separation efficiency, but the sensitivity of the method to short-chain fatty acids was quite low, causing acetic and propionic acids to be undetectable even at 100 mgL−1 in negative ESI-MS detection. Out of the four dicationic ion-pairing reagents tested, N,N’-dibutyl 1,1’-pentylenedipyrrolidium infused through a sheath-liquid ion source during CE separation was the best reagent regarding improved sensitivity and favorably complexed with anionic FAs for detection in positive ion ESI-MS. The monovalent complex showed improved ionization efficiency, providing the limits of detection (LODs) for 15 FAs ranging from 0.13 to 2.88 μg/mL and good linearity (R2 > 0.99) up to 150 μg/mL. Compared to the negative detection results, the effect was remarkable for the detection of short- and medium-chain fatty acids. The optimized CE-paired ion electrospray (PIESI)-MS method was utilized for the determination of FAs in cheese and coffee with simple pretreatment. This method may be extended for sensitive analysis of unsaturated fatty acids. © 2017 Elsevier B.V. | * |
dc.language | English | * |
dc.publisher | Elsevier B.V. | * |
dc.subject | Capillary electrophoresis-mass spectrometry (CE-MS) | * |
dc.subject | Ion paring reagent | * |
dc.subject | Paired ion electrospray ionization (PIESI) | * |
dc.subject | Saturated fatty acid | * |
dc.title | Saturated fatty acid determination method using paired ion electrospray ionization mass spectrometry coupled with capillary electrophoresis | * |
dc.type | Article | * |
dc.relation.volume | 984 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 223 | * |
dc.relation.lastpage | 231 | * |
dc.relation.journaltitle | Analytica Chimica Acta | * |
dc.identifier.doi | 10.1016/j.aca.2017.06.052 | * |
dc.identifier.wosid | WOS:000408360600021 | * |
dc.identifier.scopusid | 2-s2.0-85023172734 | * |
dc.author.google | Lee J.-H. | * |
dc.author.google | Kim S.-J. | * |
dc.author.google | Lee S. | * |
dc.author.google | Rhee J.-K. | * |
dc.author.google | Lee S.Y. | * |
dc.author.google | Na Y.-C. | * |
dc.contributor.scopusid | 이수영(53980218900;7409697278) | * |
dc.contributor.scopusid | 이진규(56599780300) | * |
dc.contributor.scopusid | 나윤철(57192691070) | * |
dc.date.modifydate | 20240415140424 | * |