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Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets

Title
Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets
Authors
Nagase K.Nagumo Y.Kim M.Kim H.-J.Kyung H.-W.Chung H.-J.Sekine H.Shimizu T.Kanazawa H.Okano T.Lee S.-J.Yamato M.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2017
Journal Title
Macromolecular Bioscience
ISSN
1616-5187JCR Link
Citation
vol. 17, no. 8
Keywords
cell sheetelectrospun fiberregenerative medicinetissue engineeringVEGF
Publisher
Wiley-VCH Verlag
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications. (Figure presented.). © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI
10.1002/mabi.201700073
Appears in Collections:
약학대학 > 약학과 > Journal papers
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