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A Metagenomic Analysis Provides a Culture-Independent Pathogen Detection for Atopic Dermatitis

Title
A Metagenomic Analysis Provides a Culture-Independent Pathogen Detection for Atopic Dermatitis
Authors
Kim, Min-HyeRho, MinaChoi, Jun-PyoChoi, Hyuh-IlPark, Han-KiSong, Woo-JungMin, Taek-KiCho, Sang-HeonCho, Young-JooKim, Yoon-KeunYang, SanghwaPyun, Bok Yang
Ewha Authors
김민혜
SCOPUS Author ID
김민혜scopus
Issue Date
2017
Journal Title
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
ISSN
2092-7355JCR Link2092-7363JCR Link
Citation
vol. 9, no. 5, pp. 453 - 461
Keywords
Atopic dermatitisextracellular vesiclemetagenomic analysis
Publisher
KOREAN ACAD ASTHMA ALLERGY & CLINICAL IMMUNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS
Abstract
Purpose: Atopic dermatitis (AD) is an inflammatory skin disease, significantly affecting the quality of life. Using AD as a model system, we tested a successive identification of AD-associated microbes, followed by a culture-independent serum detection of the identified microbe. Methods: A total of 43 genomic DNA preparations from washing fluid of the cubital fossa of 6 healthy controls, skin lesions of 27 AD patients, 10 of which later received treatment (post-treatment), were subjected to high-throughput pyrosequencing on a.Roche 454 GS-FLX platform. Results: Microbial diversity was decreased in AD, and was restored following treatment. AD was characterized by the domination of Staphylococcus, Pseudomonas, and Streptococcus, whereas Alcaligenaceae (f), Sediminibacterium, and Lactococcus were characteristic of healthy skin. An enzyme-linked immunosorbent assay (ELISA) showed that serum could be used as a source for the detection of Staphylococcus aureus extracellular vesicles (EVs). S. aureus EV-specific immunoglobulin G (IgG) and immunoglobulin E (IgE) were quantified in the serum. Conclusions: A metagenomic analysis together with a serum detection of pathogen-specific EVs provides a model for successive identification and diagnosis of pathogens of AD.
DOI
10.4168/aair.2017.9.5.453
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의료원 > 의료원 > Journal papers
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