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Antioxidant activities of ethanolic and acidic ethanolic extracts of astringent persimmon in H2O2-stimulated Caco-2 human colonic epithelial cells

Title
Antioxidant activities of ethanolic and acidic ethanolic extracts of astringent persimmon in H2O2-stimulated Caco-2 human colonic epithelial cells
Authors
Kim L.Kim Y.Kwon O.Kim J.Y.
Ewha Authors
권오란
SCOPUS Author ID
권오란scopus
Issue Date
2017
Journal Title
Food Science and Biotechnology
ISSN
1226-7708JCR Link
Citation
vol. 26, no. 4, pp. 1085 - 1091
Keywords
AntioxidantAstringent persimmonCaco-2 cellsHydrogen peroxideTannin
Publisher
The Korean Society of Food Science and Technology
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The aim of this study was to investigate the protective effects of ethanol (EAP) and acidic ethanol extracts (AEAP) of astringent persimmon fruits (Diospyros kaki) against hydrogen peroxide (H2O2)-induced oxidative stress in Caco-2 epithelial cells. Levels of reactive oxygen species (ROS) and reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein and DNA damage were measured. Treatment with H2O2 induced oxidative stress in Caco-2 cells but had no effect on SOD activity. Both EAP and AEAP protected the cells against oxidative damage and exhibited similar effects on cellular ROS scavenging activity and protein carbonyl levels. However, AEAP was more potent than EAP in normalizing CAT activity and cytosolic GSH level, and in protecting against DNA damage. These results demonstrated that astringent persimmon fruit extracts were biologically active, and that the tannin-rich fraction (AEAP) possesses stronger protective activity against H2O2-induced oxidative stress in Caco-2 cells. © 2017, The Korean Society of Food Science and Technology and Springer Science+Business Media B.V.
DOI
10.1007/s10068-017-0156-5
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
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