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Amperometric microsensors monitoring glutamate-evoked in situ responses of nitric oxide and carbon monoxide from live human neuroblastoma cells

Title
Amperometric microsensors monitoring glutamate-evoked in situ responses of nitric oxide and carbon monoxide from live human neuroblastoma cells
Authors
Ha Y.Heo C.Woo J.Ryu H.Lee Y.Suh M.
Ewha Authors
이영미
SCOPUS Author ID
이영미scopus
Issue Date
2017
Journal Title
Sensors (Switzerland)
ISSN
1424-8220JCR Link
Citation
vol. 17, no. 7
Keywords
Amperometric sensorCarbon monoxideGlutamate stimulationNeuroblastoma cellsNitric oxide
Publisher
MDPI AG
Indexed
SCIE; SCOPUS scopus
Abstract
In the brain, nitric oxide (NO) and carbon monoxide (CO) are important signaling gases which have multifaceted roles, such as neurotransmitters, neuromodulators, and vasodilators. Even though it is difficult to measure NO and CO in a living system due to their high diffusibility and extremely low release levels, electrochemical sensors are promising tools to measure in vivo and in vitro NO and CO gases. In this paper, using amperometric dual and septuple NO/CO microsensors, real-time NO and CO changes evoked by glutamate were monitored simultaneously for human neuroblastoma (SH-SY5Y) cells. In cultures, the cells were differentiated and matured into functional neurons by retinoic acid and brain-derived neurotrophic factor. When glutamate was administrated to the cells, both NO and CO increases and subsequent decreases returning to the basal levels were observed with a dual NO/CO microsensor. In order to facilitate sensor’s measurement, a flower-type septuple NO/CO microsensor was newly developed and confirmed in terms of the sensitivity and selectivity. The septuple microsensor was employed for the measurements of NO and CO changes as a function of distances from the position of glutamate injection. Our sensor measurements revealed that only functionally differentiated cells responded to glutamate and released NO and CO. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
DOI
10.3390/s17071661
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자연과학대학 > 화학·나노과학전공 > Journal papers
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