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Standardized Mori ramulus extract improves insulin secretion and insulin sensitivity in C57BLKS/J db/db mice and INS-1 cells
- Standardized Mori ramulus extract improves insulin secretion and insulin sensitivity in C57BLKS/J db/db mice and INS-1 cells
- Park S.-Y.; Jin B.; Shin J.-H.; Adisakwattana S.; Kwon O.
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- Biomedicine and Pharmacotherapy
- vol. 92, pp. 308 - 315
- Advanced glycation end-products; Hyperglycemia; Insulin resistance; Mori ramulus; Oxyresveratrol; Pancreatic β-cell dysfunction
- Elsevier Masson SAS
- SCI; SCIE; SCOPUS
- Abnormalities in the hyperbolic relationship between insulin sensitivity and insulin secretion may cause oxidative stress and non-enzymatic glycation, resulting in an increased risk of type 2 diabetes. Here, we performed a 14-week study to investigate the effects of ethanolic extract of Mori ramulus (MRE; 0, 800, and 1600 mg/kg body weight) and its signature component oxyresveratrol (OXY; 800 mg/kg body weight) on β-cell dysfunction and insulin resistance in C57BLKS/J db/db mice fed with a high-fat diet. Compared with the diabetic control group, the high-dose MRE group showed a significant decrease in fasting blood glucose (p = 0.0024); a significant increase in insulin secretion as measured by insulin (p = 0.0012) and C-peptide (p = 0.0103) levels in plasma and insulin content (p = 0.0440) and homeobox factor-1 protein expression (p = 0.0148) in the pancreas; and a significant increase in insulin sensitivity as measured by insulin receptor mRNA expression in the liver (p = 0.0179) and adipose tissue (p = 0.0491). In addition, improvements in the reactive oxygen species level and inflammatory pancreatic and hepatic tissue damage were also observed in the MRE group as assessed by histological findings. A similar but weaker effect was found in the OXY group. Furthermore, we observed a potentiating effect of MRE and OXY on insulin secretion in INS-1 cells in the presence of 27 mM glucose, together with an anti-glycation effect as indicated by methylglyoxal-trapping capacity and inhibition of advanced glycation end-product formation. Taken together, these data suggest that MRE could ameliorate β-cell dysfunction and insulin resistance by reducing oxidative damage and advanced glycation end-product (Wagenknecht et al., 2003) formation and that these effects are due, at least in part, to OXY. © 2017 Elsevier Masson SAS
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