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The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients

Title
The microbiome of the lung and its extracellular vesicles in nonsmokers, healthy smokers and COPD patients
Authors
Kim, Hyun JungKim, You-SunKim, Kang-HyunChoi, Jun-PyoKim, Yoon-KeunYun, SunmiSharma, LokeshDela Cruz, Charles S.Lee, Jae SeungOh, Yeon-MokLee, Sang-DoLee, Sei Won
Ewha Authors
김윤근
SCOPUS Author ID
김윤근scopusscopus
Issue Date
2017
Journal Title
EXPERIMENTAL AND MOLECULAR MEDICINE
ISSN
1226-3613JCR Link2092-6413JCR Link
Citation
vol. 49
Publisher
NATURE PUBLISHING GROUP
Indexed
SCI; SCIE; SCOPUS; KCI WOS
Abstract
Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease, and bacterial infection plays a role in its pathogenesis. Bacteria secrete nanometer-sized extracellular vesicles (EVs), which may induce more immune dysfunction and inflammation than the bacteria themselves. We hypothesized that the microbiome of lung EVs might have distinct characteristics depending on the presence of COPD and smoking status. We analyzed and compared the microbiomes of 13 nonsmokers with normal spirometry, 13 smokers with normal spirometry (healthy smokers) and 13 patients with COPD by using 16S ribosomal RNA gene sequencing of surgical lung tissue and lung EVs. Subjects were matched for age and sex in all groups and for smoking levels in the COPD and healthy smoker groups. Each group included 12 men and 1 woman with the same mean age of 65.5 years. In all groups, EVs consistently showed more operational taxonomic units (OTUs) than lung tissue. In the healthy smoker and COPD groups, EVs had a higher Shannon index and a lower Simpson index than lung tissue and this trend was more prominent in the COPD group. Principal component analysis (PCA) showed clusters based on sample type rather than participants' clinical characteristics. Stenotrophomonas, Propionibacterium and Alicyclobacillus were the most commonly found genera. Firmicutes were highly present in the EVs of the COPD group compared with other samples or groups. Our analysis of the lung microbiome revealed that the bacterial communities present in the EVs and in the COPD group possessed distinct characteristics with differences in the OTUs, diversity indexes and PCA clustering.
DOI
10.1038/emm.2017.7
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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