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Iloprost supports early development of in vitro-produced porcine embryos through activation of the phosphatidylinositol 3-kinase/AKT signalling pathway

Title
Iloprost supports early development of in vitro-produced porcine embryos through activation of the phosphatidylinositol 3-kinase/AKT signalling pathway
Authors
Jeong, Pil-SooYoon, Seung-BinChoi, Seon-ASong, Bong-SeokKim, Ji-SuSim, Bo-WoongPark, Young-HoYang, Hae-JunMun, Seong-EunKim, Young-HyunKang, PhilyongJeong, Kang-JinLee, YoungjeonJin, Yeung BaeHuh, Jae-WonLee, Sang-RaeKoo, Deog-BonPark, Young IlKim, Sun-UkChang, Kyu-Tae
Ewha Authors
박영일
SCOPUS Author ID
박영일scopus
Issue Date
2017
Journal Title
REPRODUCTION FERTILITY AND DEVELOPMENT
ISSN
1031-3613JCR Link1448-5990JCR Link
Citation
vol. 29, no. 7, pp. 1306 - 1318
Keywords
culture mediumsignal transduction
Publisher
CSIRO PUBLISHING
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Despite evidence of the presence of prostaglandin (PG) I-2 in mammalian oviducts, its role in early development of in vitro-produced (IVP) embryos is largely unknown. Thus, in the present study we examined the effects of iloprost, a PGI(2) analogue, on the in vitro developmental competence of early porcine embryos and the underlying mechanism(s). To examine the effects of iloprost on the development rate of IVF embryos, iloprost was added to the in vitro culture (IVC) medium and cultured for 6 days. Supplementation of the IVC medium with iloprost significantly improved developmental parameters, such as blastocyst formation rate, the trophectoderm : inner cell mass ratio and cell survival in IVF and parthenogenetically activated (PA) embryos. In addition, post-blastulation development into the expanded blastocyst stage was improved in iloprost-treated groups compared with controls. Interestingly, the phosphatidylinositol 3-kinase (PI3K)/AKT signalling pathway was significantly activated by iloprost supplementation in a concentration-dependent manner (10-1000 nM), and the beneficial effects of iloprost on the early development of porcine IVF and PA embryos was completely ablated by treatment with 2.5 mu M wortmannin, a PI3K/AKT signalling inhibitor. Importantly, expression of the PI3K/AKT signalling pathway was significantly reduced in somatic cell nuclear transfer (SCNT) compared with IVF embryos, and iloprost supported the early development of SCNT embryos, as was the case for IVF and PA embryos, suggesting a consistent effect of iloprost on the IVC of IVP porcine embryos. Together, these results indicate that iloprost can be a useful IVC supplement for production of IVP early porcine embryos with high developmental competence.
DOI
10.1071/RD15391
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신산업융합대학 > 융합콘텐츠학과 > Journal papers
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