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Design, Implementation, Simulation, and Visualization of a Highly Efficient RIM Microfluidic Mixer for Rapid Freeze-Quench of Biological Samples

Title
Design, Implementation, Simulation, and Visualization of a Highly Efficient RIM Microfluidic Mixer for Rapid Freeze-Quench of Biological Samples
Authors
Schmidt B.Mahmud G.Soh S.Kim S.H.Page T.O'Halloran T.V.Grzybowski B.A.Hoffman B.M.
Ewha Authors
김선희
Issue Date
2011
Journal Title
Applied Magnetic Resonance
ISSN
0937-9347JCR Link
Citation
vol. 40, no. 4, pp. 415 - 425
Indexed
SCI; SCIE; SCOPUS scopus
Abstract
Rapid freeze-quench (RFQ) trapping of short-lived reaction intermediates for spectroscopic study plays an important role in the characterization of biological reactions. Recently, there has been considerable effort to achieve sub-millisecond reaction deadtimes. We present here a new, robust, high-velocity microfluidic mixer that enables such rapid freeze-quenching. It is a based on the mixing method of two impinging jets commonly used in reaction injection molding of plastics. This method achieves efficient mixing by inducing chaotic flow at relatively low Reynolds numbers (Re = 140). We present the first mathematical simulation and microscopic visualization of mixing in such RFQ micromixers, the results of which show that the impinging solutions efficiently mix within the mixing chamber. These tests, along with a practical demonstration in an RFQ setup that involves copper wheels, show that this new mixer can in practice provide reaction deadtimes as low as 100 μs. © 2011 Springer-Verlag.
DOI
10.1007/s00723-011-0195-7
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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