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Foxc1 and Foxc2 in the Neural Crest Are Required for Ocular Anterior Segment Development

Title
Foxc1 and Foxc2 in the Neural Crest Are Required for Ocular Anterior Segment Development
Authors
Seo, SeungwoonChen, LishengLiu, WenzhongZhao, DeminSchultz, Kathryn M.Sasman, AmyLiu, TingZhang, Hao F.Gage, Philip J.Kume, Tsutomu
Ewha Authors
서승운
Issue Date
2017
Journal Title
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
ISSN
0146-0404JCR Link1552-5783JCR Link
Citation
vol. 58, no. 3, pp. 1368 - 1377
Keywords
corneal developmentneural crestForkheadPitx2Dkk2
Publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
PURPOSE. The large Forkhead (Fox) transcription factor family has essential roles in development, and mutations cause a wide range of ocular and nonocular disease. One member, Foxc2 is expressed in neural crest (NC)-derived periocular mesenchymal cells of the developing murine eye; however, its precise role in the development, establishment, and maintenance of the ocular surface has yet to be investigated. METHODS. To specifically delete Foxc2 from NC-derived cells, conditional knockout mice for Foxc2 (NC-Foxc2(-/-)) were generated by crossing Foxc2(F) mice with Wnt1-Cre mice. Similarly, we also generated compound NC-specific mutations of Foxc2 and a closely related gene, Foxc1 (NC-Foxc1 (/) ;NC-Foxc2 (/) ) in mice. RESULTS. Neural crest-Foxc2(-/-) mice show abnormal thickness in the peripheral-to-central corneal stroma and limbus and displaced pupils with irregular iris. The neural crest-specific mutation in Foxc2 also leads to ectopic neovascularization in the cornea, as well as impaired ocular epithelial cell identity and corneal conjunctivalization. Compound, NC-specific Foxc1; Foxc2 homozygous mutant mice have more severe defects in structures of the ocular surface, such as the cornea and eyelids, accompanied by significant declines in the expression of another key developmental factor, Pitx2, and its downstream effector Dkk2, which antagonizes canonical Wnt signaling. CONCLUSIONS. The neural crest-Foxc2 mutation is associated with corneal conjunctivalization, ectopic corneal neovascularization, and disrupted ocular epithelial cell identity. Furthermore, Foxc2 and Foxc1 cooperatively function in NC-derived mesenchymal cells to ensure proper morphogenesis of the ocular surface via the regulation of Wnt signaling. Together, Foxc2 is required in the NC lineage for mesenchymal-epithelial interactions in corneal and ocular surface development.
DOI
10.1167/iovs.16-21217
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연구기관 > 세포항상성연구센터 > Journal papers
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