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Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts
- Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts
- Yan, Xinhao; Kim, Jin Ju; Jeong, Hye Sun; Moon, Yu Kyung; Cho, Yoon Kyung; Ahn, Soyeon; Jun, Sang Beom; Kim, Hakwon; You, Youngmin
- Ewha Authors
- 유영민; 전상범
- SCOPUS Author ID
- 유영민; 전상범
- Issue Date
- Journal Title
- INORGANIC CHEMISTRY
- 0020-1669; 1520-510X
- vol. 56, no. 8, pp. 4332 - 4346
- AMER CHEMICAL SOC
- SCI; SCIE; SCOPUS
- The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control. The zinc bioimaging utility of HBO-ACR has been fully demonstrated with the use of human pancreas epidermoid carcinoma, PANC-1 cells, and rodent hippocampal neurons from cultures and acute brain slices. The results obtained through our studies established the validity of incorporating positively charged ionophores for the creation of low-affinity probes for the visualization of biometals.
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