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Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts

Title
Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts
Authors
Yan, XinhaoKim, Jin JuJeong, Hye SunMoon, Yu KyungCho, Yoon KyungAhn, SoyeonJun, Sang BeomKim, HakwonYou, Youngmin
Ewha Authors
유영민전상범
SCOPUS Author ID
유영민scopus; 전상범scopus
Issue Date
2017
Journal Title
INORGANIC CHEMISTRY
ISSN
0020-1669JCR Link1520-510XJCR Link
Citation
vol. 56, no. 8, pp. 4332 - 4346
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control. The zinc bioimaging utility of HBO-ACR has been fully demonstrated with the use of human pancreas epidermoid carcinoma, PANC-1 cells, and rodent hippocampal neurons from cultures and acute brain slices. The results obtained through our studies established the validity of incorporating positively charged ionophores for the creation of low-affinity probes for the visualization of biometals.
DOI
10.1021/acs.inorgchem.6b02786
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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