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Autophagy induction in the skeletal myogenic differentiation of human tonsil-derived mesenchymal stem cells

Title
Autophagy induction in the skeletal myogenic differentiation of human tonsil-derived mesenchymal stem cells
Authors
Park, SaeyoungChoi, YoonyoungJung, NamheeKim, JieunOh, SeiyoonYu, YeonsilAhn, Jung-HyuckJo, InhoChoi, Byung-OkJung, Sung-Chul
Ewha Authors
정성철조인호안정혁유연실박세영
SCOPUS Author ID
정성철scopus; 조인호scopus; 안정혁scopus; 유연실scopus; 박세영scopus
Issue Date
2017
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN
1107-3756JCR Link1791-244XJCR Link
Citation
vol. 39, no. 4, pp. 831 - 840
Keywords
human tonsil-derived mesenchymal stem cellsskeletal myocytetranscriptomedifferentiationautophagy
Publisher
SPANDIDOS PUBL LTD
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation and are thus a valuable source for the replacement of diseased or damaged organs. Previously, we reported that the tonsils can be an excellent reservoir of MSCs for the regeneration of skeletal muscle (SKM) damage. However, the mechanisms involved in the differentiation from tonsil-derived MSCs (T-MSCs) to myocytes via myoblasts remain unclear. To clarify these mechanisms, we analyzed gene expression profiles of T-MSCs during differentiation into myocytes compared with human skeletal muscle cells (hSKMCs). Total RNA was extracted from T-MSCs, T-MSC-derived myoblasts and myocytes, and hSKMCs and was subjected to analysis using a microarray. Microarray analysis of the three phases of myogenic differentiation identified candidate genes associated with myogenic differentiation. The expression pattern of undifferentiated T-MSCs was distinguishable from the myogenic differentiated T-MSCs and hSKMCs. In particular, we selected FNBP1L, which among the upregulated genes is essential for antibacterial autophagy, since autophagy is related to SKM metabolism and myogenesis. T-MSCs differentiated toward myoblasts and skeletal myocytes sequentially, as evidenced by increased expression of autophagy-related markers (including Beclin-1, LC3B and Atg5) and decreased expression of Bcl-2. Furthermore, we reconfirmed that autophagy has an effect on the mechanism of skeletal myogenic differentiation derived from T-MSCs by treatment with 5-azacytidine and bafilomycin A1. These data suggest that the transcriptome of the T-MSC-derived myocytes is similar to that of hSKMCs, and that autophagy has an important role in the mechanism of myogenic differentiation of T-MSCs.
DOI
10.3892/ijmm.2017.2898
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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