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Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events
- Title
- Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events
- Authors
- Yoo, Min-Sang; Shin, Minguk; Kim, Younghun; Jang, Min; Choi, Yoon-E; Park, Si Jae; Choi, Jonghoon; Lee, Jinyoung; Park, Chulhwan
- Ewha Authors
- 박시재
- SCOPUS Author ID
- 박시재
- Issue Date
- 2017
- Journal Title
- CHEMOSPHERE
- ISSN
- 0045-6535
1879-1298
- Citation
- CHEMOSPHERE vol. 175, pp. 269 - 274
- Keywords
- Bacillus subtilis; Asian dust events; Biosensor; Carbon nanotubes; Pathogen
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (10(2)-10(10) CFU/mL) and the detection limit (10(2) CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis. (C) 2017 Elsevier Ltd. All rights reserved.
- DOI
- 10.1016/j.chemosphere.2017.02.060
- Appears in Collections:
- 공과대학 > 화공신소재공학과 > Journal papers
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