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Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events

Title
Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events
Authors
Yoo, Min-SangShin, MingukKim, YounghunJang, MinChoi, Yoon-EPark, Si JaeChoi, JonghoonLee, JinyoungPark, Chulhwan
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2017
Journal Title
CHEMOSPHERE
ISSN
0045-6535JCR Link1879-1298JCR Link
Citation
vol. 175, pp. 269 - 274
Keywords
Bacillus subtilisAsian dust eventsBiosensorCarbon nanotubesPathogen
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (10(2)-10(10) CFU/mL) and the detection limit (10(2) CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis. (C) 2017 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.chemosphere.2017.02.060
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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