Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 심현보 | * |
dc.date.accessioned | 2017-04-07T01:04:08Z | - |
dc.date.available | 2017-04-07T01:04:08Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 1422-0067 | * |
dc.identifier.other | OAK-20349 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234923 | - |
dc.description.abstract | Vascular cell adhesion molecule-1 (VCAM-1) is closely associated with tumor progression and metastasis. However, the relevance and role of VCAM-1 in lung cancer have not been clearly elucidated. In this study, we found that VCAM-1 was highly overexpressed in lung cancer tissue compared with that of normal lung tissue, and high VCAM-1 expression correlated with poor survival in lung cancer patients. VCAM-1 knockdown reduced migration of A549 human lung cancer cells into Matrigel, and competitive blocking experiments targeting the Ig-like domain 6 of VCAM-1 (VCAM-1-D6) demonstrated that the VCAM-1-D6 domain was critical for VCAM-1 mediated A549 cell migration into Matrigel. Next, we developed a human monoclonal antibody specific to human and mouse VCAM-1-D6 (VCAM-1-D6 huMab), which was isolated from a human synthetic antibody library using phage display technology. Finally, we showed that VCAM-1-D6 huMab had a nanomolar affinity for VCAM-1-D6 and that it potently suppressed the migration of A549 and NCI-H1299 lung cancer cell lines into Matrigel. Taken together, these results suggest that VCAM-1-D6 is a key domain for regulating VCAM-1-mediated lung cancer invasion and that our newly developed VCAM-1-D6 huMab will be a useful tool for inhibiting VCAM-1-expressing lung cancer cell invasion. | * |
dc.language | English | * |
dc.publisher | MDPI AG | * |
dc.subject | human antibody | * |
dc.subject | invasion | * |
dc.subject | lung cancer | * |
dc.subject | Matrigel | * |
dc.subject | migration | * |
dc.subject | VCAM-1 | * |
dc.subject | VCAM-1-D6 | * |
dc.title | A Human Antibody That Binds to the Sixth Ig-Like Domain of VCAM-1 Blocks Lung Cancer Cell Migration In Vitro | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 18 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | * |
dc.identifier.doi | 10.3390/ijms18030566 | * |
dc.identifier.wosid | WOS:000396253700097 | * |
dc.identifier.scopusid | 2-s2.0-85014767130 | * |
dc.author.google | Kim, Mi Ra | * |
dc.author.google | Jang, Ji Hye | * |
dc.author.google | Park, Chang Sik | * |
dc.author.google | Kim, Taek-Keun | * |
dc.author.google | Kim, Youn-Jae | * |
dc.author.google | Chung, Junho | * |
dc.author.google | Shim, Hyunbo | * |
dc.author.google | Nam, In Hyun | * |
dc.author.google | Han, Jung Min | * |
dc.author.google | Lee, Sukmook | * |
dc.contributor.scopusid | 심현보(26635827900) | * |
dc.date.modifydate | 20240123110611 | * |