View : 148 Download: 0

Two-photon fluorescence sensors for imaging NMDA receptors and monitoring release of Zn2+ from the presynaptic terminal

Title
Two-photon fluorescence sensors for imaging NMDA receptors and monitoring release of Zn2+ from the presynaptic terminal
Authors
Chen X.Lim C.S.Lee D.Lee S.Park S.J.Kim H.M.Yoon J.
Ewha Authors
윤주영Chen Xiaoqiang
SCOPUS Author ID
윤주영scopus
Issue Date
2017
Journal Title
Biosensors and Bioelectronics
ISSN
0956-5663JCR Link
Citation
vol. 91, pp. 770 - 779
Keywords
Fluorescent probesNMDA receptorsPresynaptic terminalTwo-photon imagingZinc ions
Publisher
Elsevier Ltd
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Synaptic Zn2+ plays an important role in neurotransmission and a neuromodulator. The development of the imaging tools for monitoring spatiotemporal changes taking place in synaptic Zn2+ concentrations is necessary in order to understand the role of Zn2+ in the function of many aspects of the glutamate system. In this work, two-photon probes 1 and 2, bearing ifenprodil-like tails that have affinity for NMDA receptors of neuronal cells, were designed and prepared. The two-photon fluorescent probe 1, which bears (N-(6-acetylnaphthalen-2-yl)-N-methylglycine) as two-photon fluorophore, enables high resolution imaging of neuronal cells. The two-photon fluorescent probe 2, which contains the di-2-picolylamine (DPA) as a Zn2+-binding site, the naphthalimide unit as the two-photon fluorophore, and the ifenprodil-like tail as the NMDA receptor binding moiety, can be employed for selective detection of Zn2+ located near the NMDA receptor and for monitoring concentration changes of Zn2+ in live neurons and hippocampal tissues. © 2017 Elsevier B.V.
DOI
10.1016/j.bios.2017.01.042
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE