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beta-Catenin Accumulation Is Associated With Increased Expression of Nanog Protein and Predicts Maintenance of MSC Self-Renewal

Title
beta-Catenin Accumulation Is Associated With Increased Expression of Nanog Protein and Predicts Maintenance of MSC Self-Renewal
Authors
Yu, Sang-JinKim, Hyun-JeLee, Eui SeokPark, Chung-GyuCho, Su JinJeon, Soung-Hoo
Ewha Authors
조수진
SCOPUS Author ID
조수진scopus
Issue Date
2017
Journal Title
CELL TRANSPLANTATION
ISSN
0963-6897JCR Link1555-3892JCR Link
Citation
vol. 26, no. 2, pp. 365 - 377
Keywords
Human mesenchymal stem cells (hMSCs)Self-renewing cellsEpidermal growth factor (EGF)beta-Catenin
Publisher
COGNIZANT COMMUNICATION CORP
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Human mesenchymal stem cells (hMSCs) are self-renewing cells with the ability to differentiate into organized, functional network of cells. Recent studies have revealed that activation of the Wnt/beta-catenin pathway by a glycogen synthase kinase (GSK)-3-specific pharmacological inhibitor, Bio, results in the maintenance of self-renewal in both mouse and human ES cells. The molecular mechanism behind the maintenance of hMSCs by these factors, however, is not fully understood. We found that rEGF enhances the level of beta-catenin, a component of the Wnt/beta-catenin signaling pathway. Furthermore, it was found that beta-catenin upregulates Nanog. EGF activates the beta-catenin pathway via the Ras protein and also increased the Nanog protein and gene expression levels 2 h after rEGF treatment. These results suggest that adding EGF can enhance beta-catenin and Nanog expression in MSCs and facilitate EGF-mediated maintenance of MSC self-renewal. EGF was shown to augment MSC proliferation while preserving early progenitors within MSC population and thus did not induce differentiation. Thus, EGF not only can be used to expand MSC in vitro but also be utilized to autologous transplantation of MSCs in vivo.
DOI
10.3727/096368916X693040
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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