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Diiridium(III) complexes: luminescent probes and sensors for G-quadruplex DNA and endoplasmic reticulum imaging

Title
Diiridium(III) complexes: luminescent probes and sensors for G-quadruplex DNA and endoplasmic reticulum imaging
Authors
Anjong, Tikum FlorenceKim, GyoungmiJang, Ha YoonYoon, JuyoungKim, Jinheung
Ewha Authors
윤주영김진흥
SCOPUS Author ID
윤주영scopus; 김진흥scopus
Issue Date
2017
Journal Title
NEW JOURNAL OF CHEMISTRY
ISSN
1144-0546JCR Link1369-9261JCR Link
Citation
vol. 41, no. 1, pp. 377 - 386
Publisher
ROYAL SOC CHEMISTRY
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Two new dinuclear iridium (Ir) complexes bridged by a conjugated aromatic tppz ligand, (bhq)(2)Ir(tppz)Ir(bhq)(2) (1) and (ppy)(2)Ir(tppz)Ir(ppy)(2) (2) (bhq = benzo(h)quinolone, ppy = phenyl-pyridine, tppz = tetrapyrido[ 3,2-a:2',3'-c:3 '',2 ''-h:2''',3'''-j] phenazine), were prepared. The DNA binding properties of complexes 1 and 2 were studied using single-stranded DNA, double-stranded DNA, and G-quadruplex DNA. G-Quadruplex binding was characterized by higher emission enhancements than those observed with other control DNA strands. Photophysical properties of complexes 1 and 2 indicated that 1 displayed selective G-quadruplex binding affinity, but 2 did not. The emission properties of complexes 1 and 2 were also studied using liposomes in order to understand their possible interactions with lipid-rich organelles in live cells. The phosphorescence intensity of 1 and 2 was enhanced upon interaction with liposomes, but 2 showed greater enhancement. We also confirmed that 1 and 2 could be used as probes in live cells. Complexes 1 and 2 were intensely stained in HeLa cervical cancer cells. Excitation with a lambda = 488 nm laser showed red emission localized in the cytosol of cells. Cellular studies showed that 1 and 2 were located inside HeLa cells, but neither complex passed through the nuclear membrane. However, complexes 1 and 2 possessed superior photostability, indicating their role as good luminescent agents for imaging and tracking the endoplasmic reticulum (ER) in live cells. Overall, complex 1 exhibited better selectivity for G-quadruplexes, liposomes, and ER staining.
DOI
10.1039/c6nj02890j
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자연과학대학 > 화학·나노과학전공 > Journal papers
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