Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이동희 | - |
dc.contributor.author | 이공주 | - |
dc.date.accessioned | 2017-02-15T08:02:36Z | - |
dc.date.available | 2017-02-15T08:02:36Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.other | OAK-4184 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234426 | - |
dc.description.abstract | Angiogenesis is an essential process in physiological and pathological processes and is well-regulated to maintain the cellular homeostasis by balancing the endothelial cells in proliferation and apoptosis. Angiopoietin-1 (Ang1) regulates angiogenesis as a ligand of Tie 2 receptor tyrosine kinase. However, the regulation pathways are not well-understood. To date, only a few of the signaling molecules involved in the Tie 2 receptor tyrosine kinase-mediated angiogenesis have been identified. In this study, we systematically identified tyrosine-phosphorylated proteins in Ang1-induced signaling cascade in human umbilical vein endothelial cells (HUVECs), employing proteomic analyses combining two-dimensional gel electrophoresis. Western analysis using phosphotyrosine antibody and mass spectrometry (MALDITOF MS and nanoLC-ESI-q-TOF tandem MS). We report here the identification, semiquantitative analysis, and kinetic changes of tyrosine-phosphorylated proteins in response to Ang1 in HUVECs and identified 66 proteins among 69 protein spots showing significant changes. Of these, p54nrb was validated as a molecule involved in cell migration. These results suggest that Ang1 induces stabilization of neo-vessel network by regulating the phosphorylations of metabolic and structural proteins. © 2007 American Chemical Society. | - |
dc.language | English | - |
dc.title | Systemic analysis of tyrosine phosphorylated proteins in angiopoietin-1 induced signaling pathway of endothelial cells | - |
dc.type | Article | - |
dc.relation.issue | 8 | - |
dc.relation.volume | 6 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 3278 | - |
dc.relation.lastpage | 3290 | - |
dc.relation.journaltitle | Journal of Proteome Research | - |
dc.identifier.doi | 10.1021/pr070168k | - |
dc.identifier.wosid | WOS:000248683200035 | - |
dc.identifier.scopusid | 2-s2.0-34548175273 | - |
dc.author.google | Young M.K. | - |
dc.author.google | Seo J. | - |
dc.author.google | Yung H.K. | - |
dc.author.google | Jeong J. | - |
dc.author.google | Hye J.J. | - |
dc.author.google | Lee D.-H. | - |
dc.author.google | Gou Y.K. | - |
dc.author.google | Lee K.-J. | - |
dc.contributor.scopusid | 이동희(55460782700) | - |
dc.contributor.scopusid | 이공주(7501497635;57191532162) | - |
dc.date.modifydate | 20230411103511 | - |