Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤여준 | * |
dc.contributor.author | 박제원 | * |
dc.date.accessioned | 2017-02-15T08:02:26Z | - |
dc.date.available | 2017-02-15T08:02:26Z | - |
dc.date.issued | 2007 | * |
dc.identifier.issn | 0003-2700 | * |
dc.identifier.other | OAK-4089 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234374 | - |
dc.description.abstract | In the present study, we developed a sensitive and highly selective method of detecting the biosynthetic intermediates involved in the gentamicin pathway from a cell culture of Micromonospora echinospora. A novel extraction method utilizing a dual solid-phase extraction (SPE) technique was employed to purify and recover all of the gentamicin-related components from the cell culture broth, and high-performance liquid chromatography (HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS/MS) was used to analyze the extractant for gentamicin intermediates. The pH of the culture broth was adjusted to an acidic condition of pH 2 prior to the extraction. The samples were first cleaned with a reversed-phase AccuBOND C18 cartridge, and then the aminoglycoside components were purified using a cationic exchanger OASIS MCX cartridge. The detection limit of a gentamicin standard spiked in blank medium processed by this method was found to be approximately 5 ng for each component of the gentamicin C complex, and the mean recovery for each component of standard gentamicin was above 91% when analyzed by HPLC-ESI-MS/MS. We further demonstrated that this method enables the analytical profiling of the gentamicin-related compounds produced by wild-type M. echinospora ATCC 15835, which mainly produces the gentamicin C complex, and the UV-induced mutant strain KCTC 10506BP, which produces gentamicin B as the major product. Seven intermediates (paromamine, gentamicin A2, B, X2, A, JI-20A, and JI-20B) besides the gentamicin C complex were detected in the culture broth of both M. echinospora strains when analyzed by MS/MS for the distinct fragmentation patterns of each gentamicin component. This report displays the first example of the HPLC profiling in a wide range of structurally related biosynthetic intermediates involved in the gentamicin pathway. © 2007 American Chemical Society. | * |
dc.language | English | * |
dc.title | Analytical profiling of biosynthetic intermediates involved in the gentamicin pathway of Micromonospora echinospora by high-performance liquid chromatography using electrospray ionization mass spectrometric detection | * |
dc.type | Article | * |
dc.relation.issue | 13 | * |
dc.relation.volume | 79 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 4860 | * |
dc.relation.lastpage | 4869 | * |
dc.relation.journaltitle | Analytical Chemistry | * |
dc.identifier.doi | 10.1021/ac070028u | * |
dc.identifier.wosid | WOS:000247611600013 | * |
dc.identifier.scopusid | 2-s2.0-34447314501 | * |
dc.author.google | Je W.P. | * |
dc.author.google | Hong J.S.J. | * |
dc.author.google | Parajuli N. | * |
dc.author.google | Hwa S.K. | * |
dc.author.google | Sung R.P. | * |
dc.author.google | Lee M.-O. | * |
dc.author.google | Lim S.-K. | * |
dc.author.google | Yeo J.Y. | * |
dc.contributor.scopusid | 윤여준(7402126465) | * |
dc.date.modifydate | 20240405124244 | * |