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Bcl-XL/Bax proteins direct the fate of embryonic cortical precursor cells

Title
Bcl-XL/Bax proteins direct the fate of embryonic cortical precursor cells
Authors
Chang M.-Y.Sun W.Ochiai W.Nakashima K.Kim S.-Y.Park C.-H.Kang J.S.Shim J.-W.Jo A.-Y.Kang C.-S.Lee Y.-S.Kim J.-S.Lee S.-H.
Ewha Authors
김재상
SCOPUS Author ID
김재상scopus
Issue Date
2007
Journal Title
Molecular and Cellular Biology
ISSN
0270-7306JCR Link
Citation
vol. 27, no. 12, pp. 4293 - 4305
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
In the developing mouse brain, the highest Bcl-XL expression is seen at the peak of neurogenesis, whereas the peak of Bax expression coincides with the astrogenic period. While such observations suggest an active role of the Bcl-2 family proteins in the generation of neurons and astrocytes, no definitive demonstration has been provided to date. Using combinations of gain- and loss-of-function assays in vivo and in vitro, we provide evidence for instructive roles of these proteins in neuronal and astrocytic fate specification. Specifically, in Bax knockout mice, astrocyte formation was decreased in the developing cortices. Overexpression of Bcl-XL and Bax in embryonic cortical precursors induced neural and astrocytic differentiation, respectively, while inhibitory RNAs led to the opposite results. Importantly, inhibition of caspase activity, dimerization, or mitochondrial localization of Bcl-XL/Bax proteins indicated that the differentiation effects of Bcl-XL/Bax are separable from their roles in cell survival and apoptosis. Lastly, we describe activation of intracellular signaling pathways and expression of basic helix-loop-helix transcriptional factors specific for the Bcl-2 protein-mediated differentiation. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
DOI
10.1128/MCB.00031-07
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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