Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 한기환 | * |
dc.date.accessioned | 2017-02-15T08:02:52Z | - |
dc.date.available | 2017-02-15T08:02:52Z | - |
dc.date.issued | 2007 | * |
dc.identifier.issn | 0363-6127 | * |
dc.identifier.other | OAK-3760 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234212 | - |
dc.description.abstract | Brain/kidney (B/K) protein is a novel double C2-like-domain protein that is highly expressed in rat brain and kidney, but its cellular localization and functional role in the kidney are still undetermined. We examined the cellular localization of B/K protein in the rat kidney under normal and ischemic conditions. Ischemia-reperfusion (I/R) injury was induced by clamping both renal arteries for 45 min, and animals were killed at 1 and 6 h and 1, 2, 3, 5, 7, 14, and 28 days after the reperfusion. Kidney tissues were processed for immunohistochemistry and immunoblot analyses using rabbit anti-B/K polyclonal antibodies. In control kidneys, B/K protein was expressed primarily in distal tubules including the thick ascending limb, distal convoluted and connecting tubules, and collecting duct. Notably, B/K protein was also expressed in the straight portion (S3 segment), but not in the S1 or S2, of proximal tubules, and podocytes of the glomerulus. In rat kidneys with I/R injury, expression of B/K protein was differentially regulated according to the anatomic location. In distal tubules, overall expression of B/K protein was markedly decreased. On the other hand, I/R injury significantly increased B/K protein expression in the S3 segment of the outer medulla as well as in the rat proximal tubular epithelial cell line NRK-52E in vitro. Furthermore, B/K protein was strongly expressed in many exfoliated cells in the lumen and urine. These findings suggest that B/K protein is closely associated with cell death in proximal tubules, which are vulnerable to I/R injury in the kidney. Copyright © 2007 the American Physiological Society. | * |
dc.language | English | * |
dc.title | Differential regulation of B/K protein expression in proximal and distal tubules of rat kidneys with ischemia-reperfusion injury | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 292 | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | F100 | * |
dc.relation.lastpage | F106 | * |
dc.relation.journaltitle | American Journal of Physiology - Renal Physiology | * |
dc.identifier.doi | 10.1152/ajprenal.00009.2006 | * |
dc.identifier.wosid | WOS:000243346300013 | * |
dc.identifier.scopusid | 2-s2.0-33846249598 | * |
dc.author.google | Han K.-H. | * |
dc.author.google | Lee U.-Y. | * |
dc.author.google | Jang Y.-S. | * |
dc.author.google | Yoon M.C. | * |
dc.author.google | Young M.J. | * |
dc.author.google | Hwang I.-A. | * |
dc.author.google | Jung Y.G. | * |
dc.author.google | Lim S.-W. | * |
dc.author.google | Kim W.-Y. | * |
dc.author.google | Chul W.Y. | * |
dc.author.google | Kim J. | * |
dc.author.google | Kwon O.-J. | * |
dc.contributor.scopusid | 한기환(14622504200) | * |
dc.date.modifydate | 20240123095704 | * |