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Differential regulation of B/K protein expression in proximal and distal tubules of rat kidneys with ischemia-reperfusion injury

Title
Differential regulation of B/K protein expression in proximal and distal tubules of rat kidneys with ischemia-reperfusion injury
Authors
Han K.-H.Lee U.-Y.Jang Y.-S.Yoon M.C.Young M.J.Hwang I.-A.Jung Y.G.Lim S.-W.Kim W.-Y.Chul W.Y.Kim J.Kwon O.-J.
Ewha Authors
한기환
SCOPUS Author ID
한기환scopus
Issue Date
2007
Journal Title
American Journal of Physiology - Renal Physiology
ISSN
0363-6127JCR Link
Citation
vol. 292, no. 1, pp. F100 - F106
Indexed
SCOPUS WOS scopus
Abstract
Brain/kidney (B/K) protein is a novel double C2-like-domain protein that is highly expressed in rat brain and kidney, but its cellular localization and functional role in the kidney are still undetermined. We examined the cellular localization of B/K protein in the rat kidney under normal and ischemic conditions. Ischemia-reperfusion (I/R) injury was induced by clamping both renal arteries for 45 min, and animals were killed at 1 and 6 h and 1, 2, 3, 5, 7, 14, and 28 days after the reperfusion. Kidney tissues were processed for immunohistochemistry and immunoblot analyses using rabbit anti-B/K polyclonal antibodies. In control kidneys, B/K protein was expressed primarily in distal tubules including the thick ascending limb, distal convoluted and connecting tubules, and collecting duct. Notably, B/K protein was also expressed in the straight portion (S3 segment), but not in the S1 or S2, of proximal tubules, and podocytes of the glomerulus. In rat kidneys with I/R injury, expression of B/K protein was differentially regulated according to the anatomic location. In distal tubules, overall expression of B/K protein was markedly decreased. On the other hand, I/R injury significantly increased B/K protein expression in the S3 segment of the outer medulla as well as in the rat proximal tubular epithelial cell line NRK-52E in vitro. Furthermore, B/K protein was strongly expressed in many exfoliated cells in the lumen and urine. These findings suggest that B/K protein is closely associated with cell death in proximal tubules, which are vulnerable to I/R injury in the kidney. Copyright © 2007 the American Physiological Society.
DOI
10.1152/ajprenal.00009.2006
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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