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dc.contributor.author박혜경-
dc.contributor.author서주영-
dc.contributor.author김영주-
dc.contributor.author유경하-
dc.contributor.author우소연-
dc.date.accessioned2017-08-28T11:07:07Z-
dc.date.available2017-08-28T11:07:07Z-
dc.date.issued2007-
dc.identifier.issn0902-4441-
dc.identifier.otherOAK-3751-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/234210-
dc.description.abstractIn a previous study, we determined the gene expression profile of both megakaryocytic and non-megakaryocytic lineage cells via serial analysis of gene expression and microarray methods, and demonstrated that Pim-1 was expressed more abundantly in megakaryocytic lineage cells. In this study, we knocked down Pim-1 in K562 cells, as well as in CD34+ cells from cord blood, via RNA interference, in order to analyze the effects of Pim-1 expression on the megakaryocytic differentiation of these cells. We then additionally overexpressed the Pim-1 genes in K562 cells, and conducted a comparison of these effects with those of RNAi cells on the course of megakaryocytic differentiation. The results of this study revealed that Pim-1 knockdown exerted no effects on commitment or differentiation toward megakaryocytic lineage, as evidenced by the detected CD41+ or CD61+ cells, or on the number of megakaryocytic colony forming units. However, Pim-1 knockdown was found to elicit a reduction in CD41+ cells with >4n DNA content, and a concomitant increase in the fraction of cells achieving a ploidy of >4n in the Pim-1 overexpressing population of K562 cells. Collectively, the findings of these studies indicate that the expression of Pim-1 expression is both necessary and sufficient for polyploidization, but is not critical to cytoplasmic differentiation on megakaryopoiesis. © 2007 Blackwell Munksgaard.-
dc.languageEnglish-
dc.titlePim-1 induced polyploidy but did not affect megakaryocytic differentiation of K562 cells and CD34+ cells from cord blood-
dc.typeArticle-
dc.relation.issue2-
dc.relation.volume78-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage131-
dc.relation.lastpage138-
dc.relation.journaltitleEuropean Journal of Haematology-
dc.identifier.doi10.1111/j.1600-0609.2006.00791.x-
dc.identifier.wosidWOS:000243294800006-
dc.identifier.scopusid2-s2.0-33846083433-
dc.author.googleJung Y.-J.-
dc.author.googleChae H.-C.-
dc.author.googleSeoh J.-Y.-
dc.author.googleRyu K.-H.-
dc.author.googlePark H.-K.-
dc.author.googleKim Y.-J.-
dc.author.googleWoo S.-Y.-
dc.contributor.scopusid박혜경(36014150800)-
dc.contributor.scopusid서주영(6603709174)-
dc.contributor.scopusid김영주(55801681000)-
dc.contributor.scopusid유경하(14038236200)-
dc.contributor.scopusid우소연(7402853365)-
dc.date.modifydate20181002081005-
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의과대학 > 의학과 > Journal papers
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