Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박혜경 | * |
dc.contributor.author | 서주영 | * |
dc.contributor.author | 김영주 | * |
dc.contributor.author | 유경하 | * |
dc.contributor.author | 우소연 | * |
dc.date.accessioned | 2017-02-15T08:02:52Z | - |
dc.date.available | 2017-02-15T08:02:52Z | - |
dc.date.issued | 2007 | * |
dc.identifier.issn | 0902-4441 | * |
dc.identifier.other | OAK-3751 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234210 | - |
dc.description.abstract | In a previous study, we determined the gene expression profile of both megakaryocytic and non-megakaryocytic lineage cells via serial analysis of gene expression and microarray methods, and demonstrated that Pim-1 was expressed more abundantly in megakaryocytic lineage cells. In this study, we knocked down Pim-1 in K562 cells, as well as in CD34+ cells from cord blood, via RNA interference, in order to analyze the effects of Pim-1 expression on the megakaryocytic differentiation of these cells. We then additionally overexpressed the Pim-1 genes in K562 cells, and conducted a comparison of these effects with those of RNAi cells on the course of megakaryocytic differentiation. The results of this study revealed that Pim-1 knockdown exerted no effects on commitment or differentiation toward megakaryocytic lineage, as evidenced by the detected CD41+ or CD61+ cells, or on the number of megakaryocytic colony forming units. However, Pim-1 knockdown was found to elicit a reduction in CD41+ cells with >4n DNA content, and a concomitant increase in the fraction of cells achieving a ploidy of >4n in the Pim-1 overexpressing population of K562 cells. Collectively, the findings of these studies indicate that the expression of Pim-1 expression is both necessary and sufficient for polyploidization, but is not critical to cytoplasmic differentiation on megakaryopoiesis. © 2007 Blackwell Munksgaard. | * |
dc.language | English | * |
dc.title | Pim-1 induced polyploidy but did not affect megakaryocytic differentiation of K562 cells and CD34+ cells from cord blood | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 78 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 131 | * |
dc.relation.lastpage | 138 | * |
dc.relation.journaltitle | European Journal of Haematology | * |
dc.identifier.doi | 10.1111/j.1600-0609.2006.00791.x | * |
dc.identifier.wosid | WOS:000243294800006 | * |
dc.identifier.scopusid | 2-s2.0-33846083433 | * |
dc.author.google | Jung Y.-J. | * |
dc.author.google | Chae H.-C. | * |
dc.author.google | Seoh J.-Y. | * |
dc.author.google | Ryu K.-H. | * |
dc.author.google | Park H.-K. | * |
dc.author.google | Kim Y.-J. | * |
dc.author.google | Woo S.-Y. | * |
dc.contributor.scopusid | 박혜경(36014150800) | * |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | * |
dc.contributor.scopusid | 김영주(55801681000) | * |
dc.contributor.scopusid | 유경하(14038236200) | * |
dc.contributor.scopusid | 우소연(7402853365) | * |
dc.date.modifydate | 20240423081003 | * |