Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 최정윤 | - |
dc.date.accessioned | 2017-02-15T08:02:47Z | - |
dc.date.available | 2017-02-15T08:02:47Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | OAK-3706 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234185 | - |
dc.description.abstract | Previous studies have shown that replicative bacterial and viral DNA polymerases are able to bypass the mutagenic lesions O6-methyl and -benzyl (Bz) G. Recombinant human polymerase (pol) δ also copied past these two lesions but was totally blocked by O6-[4-oxo-4-(3-pyridyl) butyl] (Pob)G, an important mutagenic lesion formed following metabolic activation of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3- pyridyl)-1-butanone. The human translesion pols ι and κ produced mainly only 1-base incorporation opposite O6-MeG and O 6-BzG and had very low activity in copying O6-PobG. Human pol η copied past all three adducts. Steady-state kinetic analysis showed similar efficiencies of insertion opposite the O6-alkylG adducts for dCTP and dTTP with pol η and κ; pol ι showed a strong preference for dTTP. pol η, ι, and κ showed pre-steady-state kinetic bursts for dCTP incorporation opposite G and O6-MeG but little, if any, for O6-BzG or O6-PobG. Analysis of the pol η O 6-PobG products indicated that the insertion of G was opposite the base (C) 5′ of the adduct, but this product was not extended. Mass spectrometry analysis of all of the pol η primer extension products indicated multiple components, mainly with C or T inserted opposite O 6-alkylG but with no deletions in the cases of O6-MeG and O6-PobG. With pol η and O6-BzG, products were also obtained with -1 and -2 deletions and also with A inserted (opposite O 6-BzG). The results with pol η may be relevant to some mutations previously reported with O6-alkylG adducts in mammalian cells. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.language | English | - |
dc.title | Translesion synthesis across O6-alkylguanine DNA adducts by recombinant human DNA polymerases | - |
dc.type | Article | - |
dc.relation.issue | 50 | - |
dc.relation.volume | 281 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 38244 | - |
dc.relation.lastpage | 38256 | - |
dc.relation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.doi | 10.1074/jbc.M608369200 | - |
dc.identifier.wosid | WOS:000242709500017 | - |
dc.identifier.scopusid | 2-s2.0-33845971555 | - |
dc.author.google | Choi J.-Y. | - |
dc.author.google | Chowdhury G. | - |
dc.author.google | Zang H. | - |
dc.author.google | Angel K.C. | - |
dc.author.google | Vu C.C. | - |
dc.author.google | Peterson L.A. | - |
dc.author.google | Guengerich F.P. | - |
dc.contributor.scopusid | 최정윤(57223660142;34973862000) | - |
dc.date.modifydate | 20230627091252 | - |