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Expression of the ammonia transporter, Rh C glycoprotein, in normal and neoplastic human kidney

Title
Expression of the ammonia transporter, Rh C glycoprotein, in normal and neoplastic human kidney
Authors
Han K.-H.Croker B.P.Clapp W.L.Werner D.Sahni M.Kim J.Kim H.-Y.Handlogten M.E.Weiner I.D.
Ewha Authors
한기환
SCOPUS Author ID
한기환scopus
Issue Date
2006
Journal Title
Journal of the American Society of Nephrology
ISSN
1046-6673JCR Link
Citation
vol. 17, no. 10, pp. 2670 - 2679
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Recent studies have identified the presence of a novel Mep/Amt/Rh glycoprotein family of proteins that may play an important role in transmembrane ammonia transport. One of the mammalian members of this family, Rh C glycoprotein (RhCG), transports ammonia, is expressed in distal nephron sites that are critically important for ammonia secretion, exhibits increased expression in response to chronic metabolic acidosis, and originally was cloned as a tumor-related protein. The purpose of our studies was to determine the localization of RhCG in the normal and neoplastic human kidney. Immunoblot analysis of human renal cortical protein lysates demonstrated RhCG protein expression with a molecular weight of approximately 52 kD. Immunohistochemistry revealed both apical and basolateral Rhcg expression in the distal convoluted tubule, connecting segment, and initial collecting tubule and throughout the collecting duct. Co-localization with calbindin-D28k, H+-ATPase, aquaporin-2, and pendrin showed that distal convoluted tubule and connecting segment cells, A-type intercalated cells, and non-A, non-B cells express RhCG and that B-type intercalated cells, principal cells, and inner medullary collecting duct cells do not. In renal neoplasms, RhCG was expressed by chromophobe renal cell carcinoma and renal oncocytoma but not by clear cell renal cell carcinoma or by papillary renal cell carcinomas. These studies suggest that RhCG contributes to both apical and basolateral membrane ammonia transport in the human kidney. Furthermore, renal chromophobe renal cell carcinoma and renal oncocytoma seem to originate from the A-type intercalated cell. Copyright © 2006 by the American Society of Nephrology.
DOI
10.1681/ASN.2006020160
Appears in Collections:
의과대학 > 의학과 > Journal papers
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