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dc.contributor.author윤여준-
dc.date.accessioned2017-02-15T08:02:33Z-
dc.date.available2017-02-15T08:02:33Z-
dc.date.issued2006-
dc.identifier.issn0175-7598-
dc.identifier.otherOAK-3569-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/234117-
dc.description.abstractTylosin polyketide synthase (Tyl PKS) was heterologously expressed in an engineered strain of Streptomyces venezuelae bearing a deletion of pikromycin PKS gene cluster using two compatible low-copy plasmids, each under the control of a pikAI promoter. The mutant strain produced 0.5 mg/l of the 16-membered ring macrolactone, tylactone, after a 4-day culture, which is a considerably reduced culture period to reach the maximum production level compared to other Streptomyces hosts. To improve the production level of tylactone, several precursors for ethylmalonyl-CoA were fed to the growing medium, leading to a 2.8-fold improvement (1.4 mg/ml); however, switching the pikAI promoter to an actI promoter had no observable effect. In addition, a small amount of desosamine-glycosylated tylactone was detected from the extract of the mutant strain, revealing that the native glycosyltransferase DesVII displayed relaxed substrate specificity in accepting the 16-membered ring macrolactone to produce the glycosylated tylactone. These results demonstrate a successful attempt for a heterologous expression of Tyl PKS in S. venezuelae and introduce S. venezuelae as a rapid heterologous expression system for the production of secondary metabolites. © 2006 Springer-Verlag.-
dc.languageEnglish-
dc.titleHeterologous expression of tylosin polyketide synthase and production of a hybrid bioactive macrolide in Streptomyces venezuelae-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume72-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage763-
dc.relation.lastpage769-
dc.relation.journaltitleApplied Microbiology and Biotechnology-
dc.identifier.doi10.1007/s00253-006-0318-5-
dc.identifier.wosidWOS:000240809600019-
dc.identifier.scopusid2-s2.0-33748999758-
dc.author.googleJung W.S.-
dc.author.googleLee S.K.-
dc.author.googleHong J.S.J.-
dc.author.googlePark S.R.-
dc.author.googleJeong S.J.-
dc.author.googleHan A.R.-
dc.author.googleSohng J.K.-
dc.author.googleKim B.G.-
dc.author.googleChoi C.Y.-
dc.author.googleSherman D.H.-
dc.author.googleYoon Y.J.-
dc.contributor.scopusid윤여준(7402126465)-
dc.date.modifydate20200301081000-
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