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Arsenic trioxide concentration determines the fate of Ewing's sarcoma family tumors and neuroblastoma cells in vitro

Title
Arsenic trioxide concentration determines the fate of Ewing's sarcoma family tumors and neuroblastoma cells in vitro
Authors
Jung H.S.Kim H.-S.Lee M.-J.Shin H.Y.Ahn H.S.Ryu K.-H.Seoh J.-Y.Kim C.J.Jang J.J.
Ewha Authors
서주영유경하
SCOPUS Author ID
서주영scopus; 유경하scopus
Issue Date
2006
Journal Title
FEBS Letters
ISSN
0014-5793JCR Link
Citation
vol. 580, no. 20, pp. 4969 - 4975
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Arsenic trioxide (As 2O 3) induces both the differentiation and apoptosis of acute promyelocytic leukemia cells in a concentration dependent manner. We assessed the effects of As 2O 3 in CADO-ES Ewing's sarcoma (ES), JK-GMS peripheral primitive neuroectodermal tumor (PNET), and SH-SY5Y neuroblastoma cells, as they share common histogenetic backgrounds. As 2O 3 at low concentrations (0.1-1 μM) induced SH-SY5Y differentiation, and whereas PNET cells acquired a slightly differentiated phenotype, change was minimal in ES cells. Extracellular signal-regulated kinase 2 (ERK2) was activated at low As 2O 3 concentrations, and PD98059, an inhibitor of MEK-1, blocked SH-SY5Y cell differentiation by As 2O 3. High concentrations (2-10 μM) of As 2O 3 induced the apoptosis in all three cell lines, and this was accompanied by the activation of c-jun N-terminal kinase. The generation of H 2O 2 and activation of caspase 3 were identified as critical components of As 2O 3-induced apoptosis in all of the above cell lines. Fibroblast growth factor 2 enhanced As 2O 3-induced apoptosis in JK-GMS cells. The overall effects of As 2O 3 strongly suggest that it has therapeutic potential for the treatment of ES/PNET. © 2006 Federation of European Biochemical Societies.
DOI
10.1016/j.febslet.2006.07.077
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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