View : 34 Download: 0

Identification of large-scale molecular changes 1 of Autotaxin(ENPP2) knock-down by small interfering RNA in breast cancer cells

Title
Identification of large-scale molecular changes 1 of Autotaxin(ENPP2) knock-down by small interfering RNA in breast cancer cells
Authors
Noh J.H.Ryu S.Y.Eun J.W.Song J.Ahn Y.M.Kim S.Y.Lee S.H.Park W.S.Yoo N.J.Lee J.Y.Lee S.N.Nam S.W.
Ewha Authors
이시내
Issue Date
2006
Journal Title
Molecular and Cellular Biochemistry
ISSN
0300-8177JCR Link
Citation
vol. 288, no. 1-2, pp. 91 - 106
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
To understand comprehensive molecular mechanisms by which Autotaxin (ENPP2) mediates, we identified large-scale molecular changes responsible for aberrant expression of Autotaxin (ATX) on breast cancer cells by using DNA microarrays. Transcriptional over-expression of ENPP2 gene was endogenously silenced by using RNA interference technique, and then recapitulated corresponding molecular changes in MDA435 breast cancer cells. Application of nonparametric Wilcoxon statistical analyses (P<0.05) and the selection criteria of 2-fold differential gene expression change resulted in the identification of 368 genes including 133 up-regulated and 235 genes under-expressed in ENPP2-silencing MDA435 cells. Most of the functional categories of identified genes are associated with cellular metabolism, cytoskeleton organization, transcription regulation, signal transduction as well as cellular organization and biogenesis. Our data suggest that the molecular signature identified by the ENPP2-silencing methods may represent potential candidates that can explain the complicated characteristics of ATX and may serve as biomarkers, for the development of molecular-targeting therapy, in human breast cancer. © Springer Science+Business Media, Inc. 2006.
DOI
10.1007/s11010-006-9124-8
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE