Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최정윤 | - |
dc.date.accessioned | 2017-02-15T08:02:27Z | - |
dc.date.available | 2017-02-15T08:02:27Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | OAK-3510 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234091 | - |
dc.description.abstract | Heterocyclic arylamines are highly mutagenic and cause tumors in animal models. The mutagenicity is attributed to the C8- and N2-G adducts, the latter of which accumulates due to slower repair. The C 8- and N2-G adducts derived from 2-amino-3- methylimidazo[4,5-f ]quinoline (IQ) were placed at the G1 and G 3 sites of the NarI sequence, in which the G3 site is an established hot spot for frameshift mutation with the model arylamine derivative 2-acetylaminofluorene but G1 is not. Human DNA polymerase (pol) η extended primers beyond template G-IQ adducts better than did pol κ and much better than pol ι or δ. In 1-base incorporation studies, pol η inserted C and A, pol ι inserted T, and pol κ inserted G. Steady-state kinetic parameters were measured for these dNTPs opposite the C8- and N2-IQ adducts at both sites, being most favorable for pol η. Mass spectrometry of pol η extension products revealed a single major product in each of four cases; with the G1 and G 3 C8-IQ adducts, incorporation was largely error-free. With the G3 N2-IQ adduct, a -2 deletion occurred at the site of the adduct. With the G1 N2-IQ adduct, the product was error-free at the site opposite the base and then stalled. Thus, the pol η products yielded frameshifts with the N2 but not the C 8 IQ adducts. We show a role for pol η and the complexity of different chemical adducts of IQ, DNA position, and DNA polymerases. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. | - |
dc.language | English | - |
dc.title | Biochemical basis of genotoxicity of heterocyclic arylamine food mutagens: Human DNA polymerase η selectively produces a two-base deletion in copying the N2-guanyl adduct of 2-amino-3-methylimidazo[4,5-f]quinoline but not the C8 adduct at the NarI G3 site | - |
dc.type | Article | - |
dc.relation.issue | 35 | - |
dc.relation.volume | 281 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 25297 | - |
dc.relation.lastpage | 25306 | - |
dc.relation.journaltitle | Journal of Biological Chemistry | - |
dc.identifier.doi | 10.1074/jbc.M605699200 | - |
dc.identifier.wosid | WOS:000240031300031 | - |
dc.identifier.scopusid | 2-s2.0-33748746680 | - |
dc.author.google | Choi J.-Y. | - |
dc.author.google | Stover J.S. | - |
dc.author.google | Angel K.C. | - |
dc.author.google | Chowdhury G. | - |
dc.author.google | Rizzo C.J. | - |
dc.author.google | Guengerich F.P. | - |
dc.contributor.scopusid | 최정윤(57223660142;34973862000) | - |
dc.date.modifydate | 20230627091252 | - |