Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이지희 | * |
dc.contributor.author | 정영해 | * |
dc.date.accessioned | 2017-02-15T08:02:25Z | - |
dc.date.available | 2017-02-15T08:02:25Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 0300-483X | * |
dc.identifier.other | OAK-3476 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/234081 | - |
dc.description.abstract | Clarification of the key regulatory steps that lead to nuclear factor-kappa B (NF-κB) under cellular and pathological conditions is very important. The action of p38 mitogen-activated protein kinase (MAPK) on the upstream of NF-κB activation remains controversial. To examine this issue using an in vivo lung injury model, SB203580, a p38 MAPK inhibitor was given intraorally 1 h prior to lipopolysaccharide (LPS) treatment (intratracheally). The mice were sacrificed 4 h after LPS treatment. SB203580 substantially suppressed LPS-induced rises in p38 MAPK phosphorylation, neutrophil recruitment, total protein content in bronchoalveolar lavage fluid, and apoptosis of bronchoalveolar cells. Furthermore, SB203580 blocked LPS-induced NF-κB activation in lung tissue through down-regulation of serine phosphorylation, degradation of IκB-α, and consequent translocation of the p65 subunit of NF-κB to the nucleus. It is likely that, in cultured RAW 264.7 macrophages, SB203580 also blocked LPS-induced NF-κB activation in a dose-dependent manner. SB203580 inhibited LPS-induced serine phosphorylation, degradation of IκB-α, and tyrosine phosphorylation of p65 NF-κB. These data indicate that p38 MAPK acts upstream of LPS-induced NF-κB activation by modulating the phosphorylation of IκB-α and p65 NF-κB during acute lung injury. Because LPS-stimulated macrophages may contribute to inflammatory lung injury, the inhibition of the p38 MAPK-mediated intracellular signaling pathway leading to NF-κB activation represents a target for the attenuation of lung inflammation and parenchymal damage. © 2006 Elsevier Ireland Ltd. All rights reserved. | * |
dc.language | English | * |
dc.title | p38 mitogen-activated protein kinase up-regulates LPS-induced NF-κB activation in the development of lung injury and RAW 264.7 macrophages | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 225 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 36 | * |
dc.relation.lastpage | 47 | * |
dc.relation.journaltitle | Toxicology | * |
dc.identifier.doi | 10.1016/j.tox.2006.04.053 | * |
dc.identifier.wosid | WOS:000239470900004 | * |
dc.identifier.scopusid | 2-s2.0-33745854490 | * |
dc.author.google | Kim H.J. | * |
dc.author.google | Lee H.S. | * |
dc.author.google | Chong Y.H. | * |
dc.author.google | Kang J.L. | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 정영해(7201371824) | * |
dc.date.modifydate | 20240116125728 | * |