View : 172 Download: 59

Rubus occidentalis alleviates hyperalgesia induced by repeated intramuscular injection of acidic saline in rats

Title
Rubus occidentalis alleviates hyperalgesia induced by repeated intramuscular injection of acidic saline in rats
Authors
Choi, Geun JooKang, HyunKim, Won JoongBaek, Chong WhaJung, Yong HunWoo, Young CheolKwon, Ji Wung
Ewha Authors
김원중
Issue Date
2016
Journal Title
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
ISSN
1472-6882JCR Link
Citation
vol. 16
Keywords
Rubus occidentalisBlack raspberryChronic painAntinociception
Publisher
BIOMED CENTRAL LTD
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background: The purpose of this study was to evaluate the antinociceptive effect of black raspberry (Rubus occidentalis) fruit extract (ROE) in a rat model of chronic muscle pain and examine the mechanisms involved. Methods: Adult male Sprague-Dawley rats were used, and chronic muscle pain was induced by two injections of acidic saline into one gastrocnemius muscle. For the first experiment, 50 rats were randomly assigned to five groups. After the development of hyperalgesia, rats were injected intraperitoneally with 0.9 % saline or ROE (10, 30, 100, or 300 mg/kg). For the second experiment, 70 rats were randomly assigned to seven groups. Rats were injected intraperitoneally with saline, yohimbine, dexmedetomidine, prazosin, atropine, mecamylamine, or naloxone after the development of hyperalgesia. Ten minutes later, ROE (300 mg/kg) was administered intraperitoneally. For both experiments, the mechanical withdrawal threshold (MWT) was evaluated with von Frey filaments before the first acidic saline injection, 24 h after the second injection, and at 15, 30, 45, 60, 80, 100, and 120 min, 24 and 48 h after the drug administration. Results: Compared with the control group, the MWT significantly increased up to 45 min after injection of ROE 100 mg/kg and up to 60 min after injection of ROE 300 mg/kg, respectively. Injection of ROE together with yohimbine or mecamylamine significantly decreased the MWT compared with the effect of ROE alone, while ROE together with dexmedetomidine significantly increased the MWT. Conclusions: ROE showed antinociceptive activity against induced chronic muscle pain, which may be mediated by alpha 2-adrenergic and nicotinic cholinergic receptors.
DOI
10.1186/s12906-016-1192-z
Appears in Collections:
의료원 > 의료원 > Journal papers
Files in This Item:
rubus occidentalis.pdf(1.12 MB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE